Journal Article
. 2002 Aug; 441(2):159-64.
doi: 10.1007/s00428-001-0561-1.

The virtual case: a new method to completely digitize cytological and histological slides

F Demichelis 1 M Barbareschi  P Dalla Palma  S Forti  
Affiliations
  • PMID: 12189506
  •     15 citations

Abstract

The purpose of this study was to present a new method for handling histological/cytological cases. Thanks to the introduction of information technology in pathology, including the amenities afforded by robotic microscopes and digital imaging, tissue slides can be represented and evaluated using digital techniques in order to construct virtual cases through completely automated procedures. A virtual case (VC) is composed of a collection of digital images representing a histological/cytological slide at all magnification levels together with all relevant clinical data. In the present study, we describe an automated system to manage robotic microscope and image acquisition for the proper construction of VCs. These can then be viewed on a computer by means of an interface ("user-friendly") that allows one to select the more appropriate fields and to examine them at different magnifications, rapidly going from panoramic views to high resolution and vice versa. In comparison with glass slides, VCs have several advantages arising from their digital nature and can be considered a common platform for a wide range of applications such as teleconsultation, education, research, and quality control and proficiency tests.

Digital slide and virtual microscopy based routine and telepathology evaluation of routine gastrointestinal biopsy specimens.
B Molnar, L Berczi, +4 authors, Z Tulassay.
J Clin Pathol, 2003 Jun 05; 56(6). PMID: 12783970    Free PMC article.
Development and evaluation of the virtual pathology slide: a new tool in telepathology.
Sean S P Costello, Daniel J Johnston, Peter A Dervan, Daniel G O'Shea.
J Med Internet Res, 2003 Jul 15; 5(2). PMID: 12857667    Free PMC article.
A digital atlas of breast histopathology: an application of web based virtual microscopy.
M Lundin, J Lundin, H Helin, J Isola.
J Clin Pathol, 2004 Nov 26; 57(12). PMID: 15563669    Free PMC article.
Development and preliminary evaluation of the VPS ReplaySuite: a virtual double-headed microscope for pathology.
Dan J Johnston, Sean P Costello, Peter A Dervan, Daniel G O'Shea.
BMC Med Inform Decis Mak, 2005 Apr 23; 5. PMID: 15845147    Free PMC article.
[Virtual microscopy: first applications].
K Glatz-Krieger, D Glatz, M J Mihatsch.
Pathologe, 2005 Aug 13; 27(6). PMID: 16096757
Review.
Factors to keep in mind when introducing virtual microscopy.
Katharina Glatz-Krieger, Udo Spornitz, +2 authors, Dieter Glatz.
Virchows Arch, 2005 Dec 20; 448(3). PMID: 16362822
Review.
Primary histologic diagnosis using automated whole slide imaging: a validation study.
John R Gilbertson, Jonhan Ho, +3 authors, Anil V Parwani.
BMC Clin Pathol, 2006 Apr 29; 6. PMID: 16643664    Free PMC article.
[Cytology in the internet].
K Glatz, L Bubendorf, D Glatz.
Pathologe, 2007 Jul 17; 28(5). PMID: 17632721
Extended Field Laser Confocal Microscopy (EFLCM): combining automated Gigapixel image capture with in silico virtual microscopy.
Emilie Flaberg, Per Sabelström, Christer Strandh, Laszlo Szekely.
BMC Med Imaging, 2008 Jul 17; 8. PMID: 18627634    Free PMC article.
A European network for virtual microscopy--design, implementation and evaluation of performance.
Mikael Lundin, Janusz Szymas, +11 authors, Johan Lundin.
Virchows Arch, 2009 Mar 13; 454(4). PMID: 19280223
Cytologic evaluation of image-guided fine needle aspiration biopsies via robotic microscopy: A validation study.
Guoping Cai, Lisa A Teot, +4 authors, Anil V Parwani.
J Pathol Inform, 2010 Sep 02; 1. PMID: 20805959    Free PMC article.
Interactive and automated application of virtual microscopy.
Klaus Kayser, Jürgen Görtler, Stephan Borkenfeld, Gian Kayser.
Diagn Pathol, 2011 Apr 22; 6 Suppl 1. PMID: 21489181    Free PMC article.
Proliferative activity in human breast cancer: Ki-67 automated evaluation and the influence of different Ki-67 equivalent antibodies.
S Fasanella, E Leonardi, +11 authors, M Barbareschi.
Diagn Pathol, 2011 Apr 22; 6 Suppl 1. PMID: 21489202    Free PMC article.
Image Montaging for Creating a Virtual Pathology Slide: An Innovative and Economical Tool to Obtain a Whole Slide Image.
Spoorthi Ravi Banavar, Prashanthi Chippagiri, +2 authors, Premalatha Bidadi Rajashekaraiah.
Anal Cell Pathol (Amst), 2016 Oct 18; 2016. PMID: 27747147    Free PMC article.
Practical approaches to automated digital image analysis of Ki-67 labeling index in 997 breast carcinomas and causes of discordance with visual assessment.
Ah-Young Kwon, Ha Young Park, +7 authors, Eun Yoon Cho.
PLoS One, 2019 Feb 21; 14(2). PMID: 30785924    Free PMC article.