Journal Article
. 2003 Oct; 81(2):159-68.
doi: 10.1023/a:1025755717912.

Evaluation of the prognostic role of vascular endothelial growth factor and microvessel density in stages I and II breast cancer patients

V Ludovini 1 A Sidoni  L Pistola  G Bellezza  V De Angelis  S Gori  A M Mosconi  G Bisagni  R Cherubini  A Rosa Bian  C Rodinò  R Sabbatini  B Mazzocchi  E Bucciarelli  M Tonato  M Colozza  
  • PMID: 14572158
  •     19 citations


In this study, we retrospectively evaluated the expression of vascular endothelial growth factor (VEGF) and microvessel density (MVD) in 228 and 213 specimens, respectively, from stages I and II breast cancer patients (pts) enrolled in a randomized phase III adjuvant chemotherapy trial comparing epirubicin to CMF, while tamoxifen was given to all postmenopausal pts. The expression of VEGF and MVD was assessed on tissue sections formalin-fixed and paraffin-embedded by immunohistochemical staining using anti-VEGF antibody of human origin and anti-CD34 monoclonal antibody. Univariate and multivariate analysis were performed using chi squared test, log-rank test and Cox's regression model. Sixty four of 228 pts were classified as VEGF positive (28%) with no significant difference in the two treatment arms. In 213 pts evaluated for CD34, 103 pts (48%) were classified as MVD high. No significant association between VEGF and MVD was found, and neither were they correlated with many known prognostic factors such as age, tumor size, nodal status, and histological grade. The only significant correlations observed were between VEGF and estrogen receptor (ER) status (p = 0.013) and between MVD and HER2 overexpression (p = 0.023). At a median follow up of 96 months VEGF and MVD were not correlated with relapse-free survival (RFS) and overall survival (OS) in all pts and in pts assigned to one of the two treatment arms. In conclusion, VEGF and MVD retrospectively evaluated, cannot be considered prognostic factors in node negative (N-) high risk and node positive (N+) breast cancer pts treated with two different regimens of adjuvant chemotherapy.

Quantification of angiogenesis in estrogen receptor-positive and negative breast carcinoma.
Jb Parentes-Vieira, Pv Lopes-Costa, +3 authors, Bb da Silva.
Int Semin Surg Oncol, 2007 Aug 28; 4. PMID: 17718911    Free PMC article.
The determination of VEGF and MVD, among patients with primary breast cancer.
Anna Thielemann, Zygmunt Kopczyński, +3 authors, Aleksandra Baszczuk.
Pathol Oncol Res, 2008 Apr 10; 14(2). PMID: 18398702
Immunostaining with D2-40 improves evaluation of lymphovascular invasion, but may not predict sentinel lymph node status in early breast cancer.
Anna V Britto, André A Schenka, +4 authors, Laura S Ward.
BMC Cancer, 2009 Apr 10; 9. PMID: 19356249    Free PMC article.
Breast Tumor Angiogenesis and Tumor-Associated Macrophages: Histopathologist's Perspective.
Ewe Seng Ch'ng, Hasnan Jaafar, Sharifah Emilia Tuan Sharif.
Patholog Res Int, 2011 Jul 13; 2011. PMID: 21747968    Free PMC article.
Correlation of the apparent diffusion coefficiency values on diffusion-weighted imaging with prognostic factors for breast cancer.
S Y Choi, Y-W Chang, +3 authors, D Y Seo.
Br J Radiol, 2011 Dec 01; 85(1016). PMID: 22128125    Free PMC article.
Microvessel density and status of p53 protein as potential prognostic factors for adjuvant anthracycline chemotherapy in retrospective analysis of early breast cancer patients group.
Beata Biesaga, Joanna Niemiec, Marek Ziobro.
Pathol Oncol Res, 2012 May 03; 18(4). PMID: 22549273    Free PMC article.
Diffusion-weighted imaging and FDG PET/CT: predicting the prognoses with apparent diffusion coefficient values and maximum standardized uptake values in patients with invasive ductal carcinoma.
Bo Bae Choi, Sung Hun Kim, +4 authors, Hyeon Woo Yim.
World J Surg Oncol, 2012 Jun 30; 10. PMID: 22741544    Free PMC article.
The role of vascular endothelial growth factor a polymorphisms in breast cancer.
Doonyapat Sa-Nguanraksa, Pornchai O-Charoenrat.
Int J Mol Sci, 2012 Dec 04; 13(11). PMID: 23203097    Free PMC article.
Potential of Diffusion-Weighted Imaging in the Characterization of Malignant, Benign, and Healthy Breast Tissues and Molecular Subtypes of Breast Cancer.
Uma Sharma, Rani G Sah, +5 authors, Naranamangalam R Jagannathan.
Front Oncol, 2016 Jun 01; 6. PMID: 27242965    Free PMC article.
Progress in the clinical detection of heterogeneity in breast cancer.
Jun-Long Song, Chuang Chen, Jing-Ping Yuan, Sheng-Rong Sun.
Cancer Med, 2016 Oct 25; 5(12). PMID: 27774765    Free PMC article.
Dynamic Contrast-Enhanced MRI Perfusion Parameters as Imaging Biomarkers of Angiogenesis.
Sung Hun Kim, Hyeon Sil Lee, +5 authors, Ahwon Lee.
PLoS One, 2016 Dec 31; 11(12). PMID: 28036342    Free PMC article.
Correlation of Prognostic Factors of Invasive Lobular Carcinoma with ADC Value of DWI and SUVMax of FDG-PET.
Bo Bae Choi, Sung Hun Kim, Chang Suk Park, Na Young Jung.
Chonnam Med J, 2017 Jun 07; 53(2). PMID: 28584792    Free PMC article.
Apparent diffusion coefficient histogram in breast cancer brain metastases may predict their biological subtype and progression.
Sung Jun Ahn, Mijin Park, +4 authors, Jong-Min Lee.
Sci Rep, 2018 Jul 04; 8(1). PMID: 29967409    Free PMC article.
The relationship between HER2 overexpression and angiogenesis in gastric cancer.
Maciej Ciesielski, Mariusz Szajewski, +5 authors, Wiesław Janusz Kruszewski.
Medicine (Baltimore), 2018 Oct 20; 97(42). PMID: 30334990    Free PMC article.
Diffusion-Weighted Imaging of Breast Cancer: Correlation of the Apparent Diffusion Coefficient Value with Pathologic Prognostic Factors.
Şehnaz Tezcan, Nihal Uslu, +2 authors, Tugan Tezcaner.
Eur J Breast Health, 2019 Oct 18; 15(4). PMID: 31620686    Free PMC article.
Added value of mean and entropy of apparent diffusion coefficient values for evaluating histologic phenotypes of invasive ductal breast cancer with MR imaging.
Shiteng Suo, Dandan Zhang, +4 authors, Jianrong Xu.
Eur Radiol, 2018 Aug 18; 29(3). PMID: 30116958
Differentiation of Breast Lesions and Distinguishing Their Histological Subtypes Using Diffusion-Weighted Imaging and ADC Values.
Jelena Maric, Jasmina Boban, +3 authors, Dragana Bogdanovic-Stojanovic.
Front Oncol, 2020 Apr 02; 10. PMID: 32232007    Free PMC article.
Intravoxel Incoherent Motion Diffusion-Weighted Imaging for Quantitative Differentiation of Breast Tumors: A Meta-Analysis.
Jianye Liang, Sihui Zeng, +6 authors, Ni He.
Front Oncol, 2020 Nov 17; 10. PMID: 33194733    Free PMC article.
Systematic Review.
Dual-energy CT quantitative parameters for evaluating Immunohistochemical biomarkers of invasive breast cancer.
Xiaoxia Wang, Daihong Liu, +4 authors, Jiuquan Zhang.
Cancer Imaging, 2021 Jan 09; 21(1). PMID: 33413654    Free PMC article.