Journal Article
. 2009 Jan; 30(6):323-35.

Texture- and object-related automated information analysis in histological still images of various organs

Klaus Kayser 1 Sabah Amir Hoshang  Konradin Metze  Torsten Goldmann  Ekkehard Vollmer  Dominik Radziszowski  Zdravko Kosjerina  Masoud Mireskandari  Gian Kayser  
  • PMID: 19160697
  •     17 citations


Objective: To create algorithms and application tools that can support routine diagnoses of various organs.

Materials: A generalized algorithm was developed that permits the evaluation of diagnosis-associated image features obtained from hematoxylin-eosin-stained histopathologic slides. The procedure was tested for screening of tumor tissue vs. tumor-free tissue in 1,442 cases of various organs. Tissue samples studied include colon, lung, breast, pleura, stomach and thyroid. The algorithm distinguishes between texture- and object-related parameters. Texture-based information-defined as gray value per pixel measure--is independent from any segmentation procedure. It results in recursive vectors derived from time series analysis and image features obtained by spatial dependent and independent transformations. Object-based features are defined as gray value per biologic object measured.

Results: The accuracy of automated crude classification was between 95% and 100% based upon a learning set of 10 cases per diagnosis class. Results were independent from the analyzed organ. The algorithm can also distinguish between benign and malignant tumors of colon, between epithelial mesothelioma and pleural carcinomatosis or between different common pulmonary carcinomas.

Conclusion: Our algorithm distinguishes accurately among crude histologic diagnoses of various organs. It is a promising technique that can assist tissue-based diagnosis and be expanded to virtual slide evaluation.

Other Links

Image standards in tissue-based diagnosis (diagnostic surgical pathology).
Klaus Kayser, Jürgen Görtler, +3 authors, Gian Kayser.
Diagn Pathol, 2008 Apr 22; 3. PMID: 18423031    Free PMC article.
How to measure image quality in tissue-based diagnosis (diagnostic surgical pathology).
Klaus Kayser, Jürgen Görtler, +5 authors, Gian Kayser.
Diagn Pathol, 2008 Aug 05; 3 Suppl 1. PMID: 18673499    Free PMC article.
Theory of sampling and its application in tissue based diagnosis.
Klaus Kayser, Holger Schultz, +3 authors, Ekkehard Vollmer.
Diagn Pathol, 2009 Feb 18; 4. PMID: 19220904    Free PMC article.
Detection and classification of thyroid follicular lesions based on nuclear structure from histopathology images.
Wei Wang, John A Ozolek, Gustavo K Rohde.
Cytometry A, 2010 Jan 26; 77(5). PMID: 20099247    Free PMC article.
Automatic measurement of epithelium differentiation and classification of cervical intraneoplasia by computerized image analysis.
Michel Jondet, Régis Agoli-Agbo, Louis Dehennin.
Diagn Pathol, 2010 Feb 12; 5. PMID: 20148100    Free PMC article.
Quantification of virtual slides: Approaches to analysis of content-based image information.
Klaus Kayser.
J Pathol Inform, 2011 Mar 09; 2. PMID: 21383926    Free PMC article.
A supervised visual model for finding regions of interest in basal cell carcinoma images.
Ricardo Gutiérrez, Francisco Gómez, Lucía Roa-Peña, Eduardo Romero.
Diagn Pathol, 2011 Mar 31; 6. PMID: 21447178    Free PMC article.
Interactive and automated application of virtual microscopy.
Klaus Kayser, Jürgen Görtler, Stephan Borkenfeld, Gian Kayser.
Diagn Pathol, 2011 Apr 22; 6 Suppl 1. PMID: 21489181    Free PMC article.
Computer-aided classification of centroblast cells in follicular lymphoma.
Kamel Belkacem-Boussaid, Michael Pennell, +2 authors, Metin Gurcan.
Anal Quant Cytol Histol, 2011 Apr 22; 32(5). PMID: 21509147    Free PMC article.
Grid computing in image analysis.
Klaus Kayser, Jürgen Görtler, Stephan Borkenfeld, Gian Kayser.
Diagn Pathol, 2011 Apr 27; 6 Suppl 1. PMID: 21516880    Free PMC article.
Fractal characteristics of May-Grünwald-Giemsa stained chromatin are independent prognostic factors for survival in multiple myeloma.
Daniela P Ferro, Monica A Falconi, +5 authors, Konradin Metze.
PLoS One, 2011 Jun 24; 6(6). PMID: 21698234    Free PMC article.
Optimizing automated characterization of liver fibrosis histological images by investigating color spaces at different resolutions.
Doaa Mahmoud-Ghoneim.
Theor Biol Med Model, 2011 Jul 16; 8. PMID: 21756305    Free PMC article.
Computerized texture analysis of atypical immature myeloid precursors in patients with myelodysplastic syndromes: an entity between blasts and promyelocytes.
Joyce R Vido, Randall L Adam, Irene G H Lorand-Metze, Konradin Metze.
Diagn Pathol, 2011 Oct 01; 6. PMID: 21958512    Free PMC article.
Virtual slides in peer reviewed, open access medical publication.
Klaus Kayser, Stephan Borkenfeld, Torsten Goldmann, Gian Kayser.
Diagn Pathol, 2011 Dec 21; 6. PMID: 22182763    Free PMC article.
The history of pathology informatics: A global perspective.
Seung Park, Anil V Parwani, +15 authors, Liron Pantanowitz.
J Pathol Inform, 2013 Jul 23; 4. PMID: 23869286    Free PMC article.
Follicular thyroid lesions: is there a discriminatory potential in the computerized nuclear analysis?
Flávia O Valentim, Bárbara P Coelho, +8 authors, Gláucia M F S Mazeto.
Endocr Connect, 2018 Jul 06; 7(8). PMID: 29973373    Free PMC article.
Role of artificial intelligence in diagnostic oral pathology-A modern approach.
Ayinampudi Bhargavi Krishna, Azra Tanveer, Pancha Venkat Bhagirath, Ashalata Gannepalli.
J Oral Maxillofac Pathol, 2020 Jun 09; 24(1). PMID: 32508465    Free PMC article.