Journal Article
. 2009 Aug; 4:27.
doi: 10.1186/1746-1596-4-27.

The human placenta releases substances that drive lung cancer into apoptosis

Sebastian Marwitz 1 Tobias Zeiser  Holger Schultz  Daniel Kähler  Mahdi Abdullah  Hans-Peter Hauber  Peter Zabel  Ekkehard Vollmer  Torsten Goldmann  
  • PMID: 19698096
  •     12 References
  •     1 citations


Background: As there is no optimal treatment of non small cell lung cancer due to its resistance to common chemotherapeutics, we investigated the effect of human placenta-conditioned medium on tumor tissue. The human placenta constitutes a mixture of maternal and fetal origin and displays a variety of immunomodulatory aspects.

Methods: Freshly resected non small cell lung cancer tissues were incubated with placenta-conditioned medium in a short-term tissue culture model and A549 cells were challenged, respectively. Term placenta was used for producing conditioned medium and HOPE-fixed stimulated tumor tissue was analyzed for expression of caspase-3 and Ki67 via immunohistochemistry. The effects of conditioned medium on squamous cell carcinoma were further compared to physiological concentrations of Carboplat/Gemzar.

Results: Conditioned medium caused in 2 of 3 cases elevated expression of caspase-3 and reduced expression of Ki67 in 3 out of 3 cases, while the chemotherapeutic agents caused no comparable expression of caspase-3 or reduction of Ki67. In cell culture up to 50% of karyopyknosis was investigated and even sterile-filtrated medium caused widespread reduction of Ki67 on protein level.

Conclusion: Human placenta releases substances that mediate apoptosis and reduce proliferation in tumor tissue and cell culture. As even sterile-filtrated medium caused the mentioned effects we hypothesize one or more soluble mediators. The detailed way of promoting apoptosis and nature of these mediators need to be elucidated in further studies.

TKTL1 is overexpressed in a large portion of non-small cell lung cancer specimens.
Holger Schultz, Daniel Kähler, +3 authors, Torsten Goldmann.
Diagn Pathol, 2008 Aug 14; 3. PMID: 18700018    Free PMC article.
Death-inducing tumour necrosis factor (TNF) superfamily ligands and receptors are transcribed in human placentae, cytotrophoblasts, placental macrophages and placental cell lines.
T A Phillips, J Ni, J S Hunt.
Placenta, 2001 Oct 13; 22(8-9). PMID: 11597186
[Effect of decidual cell conditioned media on invasion-related gene expression of ovarian tumor cell line COC1].
Jin-hu Zhu, Zhan-jun Pang, Shi-ling Chen, Fu-qi Xing.
Di Yi Jun Yi Da Xue Xue Bao, 2004 Jul 20; 24(7). PMID: 15257893
Stranger in a strange land.
Joan S Hunt.
Immunol Rev, 2006 Sep 16; 213. PMID: 16972895    Free PMC article.
A novel human ex vivo model for the analysis of molecular events during lung cancer chemotherapy.
Dagmar S Lang, Daniel Droemann, +6 authors, Torsten Goldmann.
Respir Res, 2007 Jun 15; 8. PMID: 17567922    Free PMC article.
Gene expression patterns in human placenta.
Ruchira Sood, James L Zehnder, Maurice L Druzin, Patrick O Brown.
Proc Natl Acad Sci U S A, 2006 Mar 29; 103(14). PMID: 16567644    Free PMC article.
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Growth suppression of transformed cells by a human placental extract not related to transforming growth factor beta.
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