Journal Article
. 2011 Feb; 104(6):1007-12.
doi: 10.1038/bjc.2011.33.

Expression of osteopontin coregulators in primary colorectal cancer and associated liver metastases

D J Mole 1 C O'Neill  P Hamilton  B Olabi  V Robinson  L Williams  T Diamond  M El-Tanani  F C Campbell  
Affiliations
  • PMID: 21343932
  •     19 References
  •     10 citations

Abstract

Background: A transcription regulatory complex (TRC) that includes Ets1, Ets2, PEA3 and β-catenin/T-cell factors regulates osteopontin (OPN) that is implicated in colorectal cancer (CRC) dissemination. The consistency of OPN transcriptional control between primary CRC and metastases is unclear. This study investigates expression and prognostic significance of the OPN-TRC in primary human CRC and associated colorectal liver metastases (CRLM).

Methods: Osteopontin-TRC factors were assayed by digital microscopy in 38 primary CRCs and matched CRLM specimens and assessed against clinical prognosis.

Results: In primary CRC, OPN expression intensity correlated with that of its co-activators, PEA3 (r=0.600; P<0.01), Ets1 (r=0.552; P<0.01), Ets2 (r=0.521; P<0.01) and had prognostic significance. Osteopontin intensity in primary CRC inversely correlated with the interval between diagnosis and resection of CRLM. Overall OPN intensity was lower in CRLM than primary CRC and correlations with co-activators were weaker, for example, Ets1 (P=0.047), PEA3 (P=0.022) or nonsignificant (Ets2). The ratio of OPN expression in CRLM vs primary CRC had prognostic significance.

Conclusion: This study supports transcriptional control of OPN by known coregulators in both primary and secondary CRC. Weaker associations in CRLM suggest involvement of other unknown factors possibly from the liver microenvironment or resulting from additional genetic or epigenetic changes that drive tumour metastatic capability in OPN transcriptional control.

HER2 amplification status in breast cancer: a comparison between immunohistochemical staining and fluorescence in situ hybridisation using manual and automated quantitative image analysis scoring techniques.
C M Ellis, M J Dyson, T J Stephenson, E L Maltby.
J Clin Pathol, 2005 Jun 25; 58(7). PMID: 15976337    Free PMC article.
Polyoma enhancer activator 3, an ets transcription factor, mediates the induction of cyclooxygenase-2 by nitric oxide in colorectal cancer cells.
Yongmin Liu, Gregory L Borchert, James M Phang.
J Biol Chem, 2004 Feb 21; 279(18). PMID: 14976201
Factors influencing the natural history of colorectal liver metastases.
R Stangl, A Altendorf-Hofmann, R M Charnley, J Scheele.
Lancet, 1994 Jun 04; 343(8910). PMID: 7515134
Highly Cited.
Critical determinants of metastasis.
Isaiah J Fidler.
Semin Cancer Biol, 2002 May 25; 12(2). PMID: 12027580
Highly Cited. Review.
Ets gene PEA3 cooperates with beta-catenin-Lef-1 and c-Jun in regulation of osteopontin transcription.
Mohamed El-Tanani, Angela Platt-Higgins, Philip S Rudland, Frederick Charles Campbell.
J Biol Chem, 2004 Mar 03; 279(20). PMID: 14990565
Development and evaluation of the virtual pathology slide: a new tool in telepathology.
Sean S P Costello, Daniel J Johnston, Peter A Dervan, Daniel G O'Shea.
J Med Internet Res, 2003 Jul 15; 5(2). PMID: 12857667    Free PMC article.
Gene expression profile of metastatic human pancreatic cancer cells depends on the organ microenvironment.
Toru Nakamura, Isaiah J Fidler, Kevin R Coombes.
Cancer Res, 2007 Jan 11; 67(1). PMID: 17210693
Application of virtual microscopy in clinical cytopathology.
D M Steinberg, S Z Ali.
Diagn Cytopathol, 2001 Dec 18; 25(6). PMID: 11747236
Interferon-induced transmembrane 3 binds osteopontin in vitro: expressed in vivo IFITM3 reduced OPN expression.
M K El-Tanani, D Jin, F C Campbell, P G Johnston.
Oncogene, 2009 Nov 11; 29(5). PMID: 19901966
Molecular staging for survival prediction of colorectal cancer patients.
Steven Eschrich, Ivana Yang, +9 authors, Timothy J Yeatman.
J Clin Oncol, 2005 May 24; 23(15). PMID: 15908663
Highly Cited.
The ets protein PEA3 suppresses HER-2/neu overexpression and inhibits tumorigenesis.
X Xing, S C Wang, +8 authors, M C Hung.
Nat Med, 2000 Feb 02; 6(2). PMID: 10655108
The regulation and role of osteopontin in malignant transformation and cancer.
Mohamed K El-Tanani, Frederick Charles Campbell, +3 authors, Philip S Rudland.
Cytokine Growth Factor Rev, 2006 Nov 23; 17(6). PMID: 17113338
Review.
Transcriptional regulation of osteopontin gene in vivo by PEBP2alphaA/CBFA1 and ETS1 in the skeletal tissues.
M Sato, E Morii, +11 authors, S Nomura.
Oncogene, 1998 Oct 30; 17(12). PMID: 9794229
Guidelines for resection of colorectal cancer liver metastases.
O J Garden, M Rees, +5 authors, R W Parks.
Gut, 2006 Jul 13; 55 Suppl 3. PMID: 16835351    Free PMC article.
Highly Cited.
Osteopontin identified as lead marker of colon cancer progression, using pooled sample expression profiling.
Deepak Agrawal, Tingan Chen, +6 authors, Timothy J Yeatman.
J Natl Cancer Inst, 2002 Apr 04; 94(7). PMID: 11929952
Highly Cited.
Activation of beta-catenin-Tcf signaling in colon cancer by mutations in beta-catenin or APC.
P J Morin, A B Sparks, +4 authors, K W Kinzler.
Science, 1997 Mar 21; 275(5307). PMID: 9065402
Highly Cited.
Osteopontin regulates multiple functions contributing to human colon cancer development and progression.
R B Irby, S M McCarthy, T J Yeatman.
Clin Exp Metastasis, 2005 Feb 01; 21(6). PMID: 15679049
Digital slide and virtual microscopy based routine and telepathology evaluation of routine gastrointestinal biopsy specimens.
B Molnar, L Berczi, +4 authors, Z Tulassay.
J Clin Pathol, 2003 Jun 05; 56(6). PMID: 12783970    Free PMC article.
Wnt signaling and cancer.
P Polakis.
Genes Dev, 2000 Aug 02; 14(15). PMID: 10921899
Highly Cited. Review.
The heterotrimeric laminin coiled-coil domain exerts anti-adhesive effects and induces a pro-invasive phenotype.
Patricia Santos-Valle, Irene Guijarro-Muñoz, +6 authors, Luis Alvarez-Vallina.
PLoS One, 2012 Jun 23; 7(6). PMID: 22723936    Free PMC article.
Biological resonance for cancer metastasis, a new hypothesis based on comparisons between primary cancers and metastases.
Dongwei Gao, Sha Li.
Cancer Microenviron, 2013 Nov 12; 6(3). PMID: 24214411    Free PMC article.
Pea3 expression promotes the invasive and metastatic potential of colorectal carcinoma.
Aruz Mesci, Samira Taeb, +3 authors, Stanley K Liu.
World J Gastroenterol, 2014 Dec 18; 20(46). PMID: 25516649    Free PMC article.
Post-operative plasma osteopontin predicts distant metastasis in human colorectal cancer.
Lui Ng, Timothy Ming-Hun Wan, +14 authors, Wai-Lun Law.
PLoS One, 2015 May 12; 10(5). PMID: 25961724    Free PMC article.
Relationship between osteopontin and β-catenin immunohistochemical expression and prognostic parameters of colorectal carcinoma.
Nermeen S Youssef, Wesam M Osman.
Int J Clin Exp Pathol, 2015 May 15; 8(2). PMID: 25973035    Free PMC article.
Regulatory activity based risk model identifies survival of stage II and III colorectal carcinoma.
Gang Liu, Chuanpeng Dong, +5 authors, Lei Liu.
Oncotarget, 2017 Dec 13; 8(58). PMID: 29228695    Free PMC article.
Circulating Levels of Osteopontin Predict Patients' Outcome after Resection of Colorectal Liver Metastases.
Sven H Loosen, Daniel Heise, +7 authors, Marcel Binnebösel.
J Clin Med, 2018 Oct 31; 7(11). PMID: 30373147    Free PMC article.
Osteopontin expression is associated with progression and adverse prognosis in patients with resectable gastrointestinal stromal tumor.
Jinhai Wang, Xiaobin Cheng, Sen Lu, Weina Fan.
Int J Clin Exp Pathol, 2020 Jan 15; 12(4). PMID: 31933953    Free PMC article.
Therapeutic Ablation of Gain-of-Function Mutant p53 in Colorectal Cancer Inhibits Stat3-Mediated Tumor Growth and Invasion.
Ramona Schulz-Heddergott, Nadine Stark, +7 authors, Ute M Moll.
Cancer Cell, 2018 Aug 15; 34(2). PMID: 30107178    Free PMC article.
Highly Cited.
IS THERE A CLINICAL PATHOLOGICAL CORRELATION OF COLORECTAL ADENOCARCINOMA WITH THE IMMUNOHISTOCHEMICAL EXPRESSION OF OPN AND ABCB5?
Diogo Francesco Castoldi, Osvaldo Malafaia, +7 authors, Carmen Australia Paredes Marcondes Ribas.
Arq Bras Cir Dig, 2021 Mar 25; 33(4). PMID: 33759959    Free PMC article.