Journal Article
. 2011 Apr; 6 Suppl 1:S10.
doi: 10.1186/1746-1596-6-S1-S10.

Interactive and automated application of virtual microscopy

Klaus Kayser 1 Jürgen Görtler  Stephan Borkenfeld  Gian Kayser  
  • PMID: 21489181
  •     24 References
  •     8 citations


Virtual microscopy can be applied in an interactive and an automated manner. Interactive application is performed in close association to conventional microscopy. It includes image standardization suitable to the performance of an individual pathologist such as image colorization, white color balance, or individual adjusted brightness. The steering commands have to include selection of wanted magnification, easy navigation, notification, and simple measurements (distances, areas). The display of the histological image should be adjusted to the physical limits of the human eye, which are determined by a view angle of approximately 35 seconds. A more sophisticated performance should include acoustic commands that replace the corresponding visual commands. Automated virtual microscopy includes so-called microscopy assistants which can be defined similar to the developed assistants in computer based editing systems (Microsoft Word, etc.). These include an automated image standardization and correction algorithms that excludes images of poor quality (for example uni-colored or out-of-focus images), an automated selection of the most appropriate field of view, an automated selection of the best magnification, and finally proposals of the most probable diagnosis. A quality control of the final diagnosis, and feedback to the laboratory determine the proposed system. The already developed tools of such a system are described in detail, as well as the results of first trials. In order to enhance the speed of such a system, and to allow further user-independent development a distributed implementation probably based upon Grid technology seems to be appropriate. The advantages of such a system as well as the present pathology environment and its expectations will be discussed in detail.

The use of digital 'virtual slides' in the quality assessment of haematological morphology: results of a pilot exercise involving UK NEQAS(H) participants.
J Burthem, M Brereton, +9 authors, K Hyde.
Br J Haematol, 2005 Jul 21; 130(2). PMID: 16029459
A European network for virtual microscopy--design, implementation and evaluation of performance.
Mikael Lundin, Janusz Szymas, +11 authors, Johan Lundin.
Virchows Arch, 2009 Mar 13; 454(4). PMID: 19280223
Web-based virtual microscopy at the RWTH Aachen University: didactic concept, methods and analysis of acceptance by the students.
Magdalene Merk, Ruth Knuechel, Alberto Perez-Bouza.
Ann Anat, 2010 Apr 07; 192(6). PMID: 20363112
Telepathology and continuous education: important tools for pathologists of developing countries.
Hugo Góngora Jará, Héctor A Barcelo.
Diagn Pathol, 2008 Aug 05; 3 Suppl 1. PMID: 18673513    Free PMC article.
Virtual microscopy and digital cytology: state of the art.
Daniele Giansanti, Mauro Grigioni, +4 authors, Maria Rosaria Giovagnoli.
Ann Ist Super Sanita, 2010 Jun 23; 46(2). PMID: 20567061
The use of virtual microscopy for proficiency testing in gynecologic cytopathology: a feasibility study using ScanScope.
Alberto M Marchevsky, Renu Khurana, +3 authors, Shika Bose.
Arch Pathol Lab Med, 2006 Mar 08; 130(3). PMID: 16519563
Virtual microscopy in pathology education.
Fred R Dee.
Hum Pathol, 2009 Jun 23; 40(8). PMID: 19540551
Histology, imaging and new diagnostic work-flows in pathology.
John Gilbertson, Yukako Yagi.
Diagn Pathol, 2008 Aug 05; 3 Suppl 1. PMID: 18673502    Free PMC article.
Theory of sampling and its application in tissue based diagnosis.
Klaus Kayser, Holger Schultz, +3 authors, Ekkehard Vollmer.
Diagn Pathol, 2009 Feb 18; 4. PMID: 19220904    Free PMC article.
AI (artificial intelligence) in histopathology--from image analysis to automated diagnosis.
Klaus Kayser, Jürgen Görtler, +4 authors, Gian Kayser.
Folia Histochem Cytobiol, 2010 Feb 19; 47(3). PMID: 20164018
From telepathology to virtual pathology institution: the new world of digital pathology.
K Kayser, G Kayser, D Radziszowski, A Oehmann.
Rom J Morphol Embryol, 2005 Apr 26; 45. PMID: 15847374
Automated primary screening devices. Expectations for the next generation.
P H Bartels.
Acta Cytol, 2000 Oct 04; 44(5). PMID: 11015970
From virtual microscopy to systems pathology: a meeting report of the 1st European workshop on tissue imaging and analysis, Heidelberg, Germany, 13-14 February 2009.
Niels Grabe, Peter Schirmacher.
Virchows Arch, 2009 Aug 05; 455(2). PMID: 19652999
Object orientated automated image analysis: quantitative and qualitative estimation of inflammation in mouse lung.
Coralie Apfeldorfer, Kristina Ulrich, +4 authors, Virgile Richard.
Diagn Pathol, 2008 Aug 05; 3 Suppl 1. PMID: 18673504    Free PMC article.
New developments in digital pathology: from telepathology to virtual pathology laboratory.
Klaus Kayser, Gian Kayser, Dominik Radziszowski, Alexander Oehmann.
Stud Health Technol Inform, 2005 Feb 19; 105. PMID: 15718595
Digitized pathology: theory and experiences in automated tissue-based virtual diagnosis.
K Kayser, D Radziszowski, +2 authors, G Kayser.
Rom J Morphol Embryol, 2006 Jul 14; 47(1). PMID: 16838053
Virtual microscopy and grid-enabled decision support for large-scale analysis of imaged pathology specimens.
Lin Yang, Wenjin Chen, +4 authors, David J Foran.
IEEE Trans Inf Technol Biomed, 2009 Apr 17; 13(4). PMID: 19369162    Free PMC article.
Web-based virtual microscopy in teaching and standardizing Gleason grading.
Henrik Helin, Mikael Lundin, +5 authors, Jorma Isola.
Hum Pathol, 2005 May 14; 36(4). PMID: 15891999
Automated region of interest retrieval and classification using spectral analysis.
Myriam Oger, Philippe Belhomme, +2 authors, Abderrahim Elmoataz.
Diagn Pathol, 2008 Aug 05; 3 Suppl 1. PMID: 18673505    Free PMC article.
Innovations in medical imaging and virtual microscopy.
Ronald S Weinstein.
Hum Pathol, 2005 May 14; 36(4). PMID: 15891989
Theory and implementation of an electronic, automated measurement system for images obtained from immunohistochemically stained slides.
Gian Kayser, Dominik Radziszowski, +2 authors, Klaus Kayser.
Anal Quant Cytol Histol, 2006 Mar 29; 28(1). PMID: 16566277
The virtual case: a new method to completely digitize cytological and histological slides.
F Demichelis, M Barbareschi, P Dalla Palma, S Forti.
Virchows Arch, 2002 Aug 22; 441(2). PMID: 12189506
Virtual microscopy for learning and assessment in pathology.
Rakesh K Kumar, Gary M Velan, +3 authors, Denis Wakefield.
J Pathol, 2004 Oct 08; 204(5). PMID: 15470688
Texture- and object-related automated information analysis in histological still images of various organs.
Klaus Kayser, Sabah Amir Hoshang, +6 authors, Gian Kayser.
Anal Quant Cytol Histol, 2009 Jan 24; 30(6). PMID: 19160697
Computerized texture analysis of atypical immature myeloid precursors in patients with myelodysplastic syndromes: an entity between blasts and promyelocytes.
Joyce R Vido, Randall L Adam, Irene G H Lorand-Metze, Konradin Metze.
Diagn Pathol, 2011 Oct 01; 6. PMID: 21958512    Free PMC article.
Introduction of virtual microscopy in routine surgical pathology--a hypothesis and personal view from Europe.
Klaus Kayser.
Diagn Pathol, 2012 May 02; 7. PMID: 22546238    Free PMC article.
CognitionMaster: an object-based image analysis framework.
Stephan Wienert, Daniel Heim, +8 authors, Frederick Klauschen.
Diagn Pathol, 2013 Mar 01; 8. PMID: 23445542    Free PMC article.
Clinical validation of the gastrointestinal NET grading system: Ki67 index criteria of the WHO 2010 classification is appropriate to predict metastasis or recurrence.
Takeshi Yamaguchi, Takahiro Fujimori, +5 authors, Hiroyuki Kato.
Diagn Pathol, 2013 Apr 24; 8. PMID: 23607525    Free PMC article.
The history of pathology informatics: A global perspective.
Seung Park, Anil V Parwani, +15 authors, Liron Pantanowitz.
J Pathol Inform, 2013 Jul 23; 4. PMID: 23869286    Free PMC article.
Relationship between magnification and resolution in digital pathology systems.
Tiffany L Sellaro, Robert Filkins, +5 authors, Michael Montalto.
J Pathol Inform, 2013 Oct 02; 4. PMID: 24083056    Free PMC article.
Quantification of myocardial fibrosis by digital image analysis and interactive stereology.
Dainius Daunoravicius, Justinas Besusparis, +8 authors, Arvydas Laurinavicius.
Diagn Pathol, 2014 Jun 11; 9. PMID: 24912374    Free PMC article.
Curvelet based offline analysis of SEM images.
Syed Hamad Shirazi, Nuhman ul Haq, +2 authors, Ihsan ul Haque.
PLoS One, 2014 Aug 05; 9(8). PMID: 25089617    Free PMC article.