Journal Article
. 2012 May; 3:20.
doi: 10.4103/2153-3539.95456.

Computer-assisted imaging algorithms facilitate histomorphometric quantification of kidney damage in rodent renal failure models

Marcin Klapczynski 1 Gerard D Gagne  Sherry J Morgan  Kelly J Larson  Bruce E Leroy  Eric A Blomme  Bryan F Cox  Eugene W Shek  
Affiliations
  • PMID: 22616032
  •     19 References
  •     15 citations

Abstract

Introduction: Surgical 5/6 nephrectomy and adenine-induced kidney failure in rats are frequently used models of progressive renal failure. In both models, rats develop significant morphological changes in the kidneys and quantification of these changes can be used to measure the efficacy of prophylactic or therapeutic approaches. In this study, the Aperio Genie Pattern Recognition technology, along with the Positive Pixel Count, Nuclear and Rare Event algorithms were used to quantify histological changes in both rat renal failure models.

Methods: Analysis was performed on digitized slides of whole kidney sagittal sections stained with either hematoxylin and eosin or immunohistochemistry with an anti-nestin antibody to identify glomeruli, regenerating tubular epithelium, and tubulointerstitial myofibroblasts. An anti-polymorphonuclear neutrophil (PMN) antibody was also used to investigate neutrophil tissue infiltration.

Results: Image analysis allowed for rapid and accurate quantification of relevant histopathologic changes such as increased cellularity and expansion of glomeruli, renal tubular dilatation, and degeneration, tissue inflammation, and mineral aggregation. The algorithms provided reliable and consistent results in both control and experimental groups and presented a quantifiable degree of damage associated with each model.

Conclusion: These algorithms represent useful tools for the uniform and reproducible characterization of common histomorphologic features of renal injury in rats.

Keywords: 5/6 nephrectomy; Genie; adenine kidney model; digital pathology; digital slide; image analysis; rodent renal failure model.

Quantification of interstitial fibrosis by image analysis on routine renal biopsy in patients receiving cyclosporine.
Aude Servais, Vannary Meas-Yedid, +5 authors, Eric Thervet.
Transplantation, 2008 Jan 01; 84(12). PMID: 18165770
Systemic disorders of calcium dynamics in rats with adenine-induced renal failure: implication for chronic kidney disease-related complications.
Ryuji Ikeda, Yasuo Imai, Wakako Maruyama, Kazushige Mizoguchi.
Nephrology (Carlton), 2010 Apr 10; 15(1). PMID: 20377772
Accuracy of a remote quantitative image analysis in the whole slide images.
Janina Słodkowska, Tomasz Markiewicz, +4 authors, Piotr Murawski.
Diagn Pathol, 2011 Apr 22; 6 Suppl 1. PMID: 21489191    Free PMC article.
Microvascular density as an independent predictor of clinical outcome in renal cell carcinoma: an automated image analysis study.
Vladimir V Iakovlev, Manal Gabril, +11 authors, George M Yousef.
Lab Invest, 2011 Nov 02; 92(1). PMID: 22042086
High-calcium vs high-phosphate intake and small artery tone in advanced experimental renal insufficiency.
Peeter Kööbi, Tuija I Vehmas, +9 authors, Ilkka Pörsti.
Nephrol Dial Transplant, 2006 Jul 14; 21(10). PMID: 16837509
Practice evolution: decentralized computer-assisted immunohistochemical image analysis.
Richard C Friedberg, Liron Pantanowitz.
Arch Pathol Lab Med, 2009 Apr 28; 133(4). PMID: 19391658
New computerized color image analysis for the quantification of interstitial fibrosis in renal transplantation.
Vannary Meas-Yedid, Aude Servais, +9 authors, Emmanuel Morelon.
Transplantation, 2011 Sep 20; 92(8). PMID: 21926945
Nestin expression in the kidney with an obstructed ureter.
T Sakairi, K Hiromura, +9 authors, Y Nojima.
Kidney Int, 2007 Apr 13; 72(3). PMID: 17429339
Chronic kidney disease: detection and evaluation.
Margaret Baumgarten, Todd Gehr.
Am Fam Physician, 2011 Nov 17; 84(10). PMID: 22085668
Review.
A progressive glomerulosclerosis occurring in partial five-sixths nephrectomized rats.
T Shimamura, A B Morrison.
Am J Pathol, 1975 Apr 01; 79(1). PMID: 164779    Free PMC article.
Highly Cited.
Animal model of adenine-induced chronic renal failure in rats.
T Yokozawa, P D Zheng, H Oura, F Koizumi.
Nephron, 1986 Jan 01; 44(3). PMID: 3785486
Highly Cited.
Hyperphosphatemia-induced hyperparathyroidism in 5/6 nephrectomized rats: development of a new animal model.
Ying Jiang, Mei Wang.
Chin Med J (Engl), 2008 Dec 24; 121(23). PMID: 19102965
Review of the current state of whole slide imaging in pathology.
Liron Pantanowitz, Paul N Valenstein, +5 authors, Terence J Colgan.
J Pathol Inform, 2011 Sep 03; 2. PMID: 21886892    Free PMC article.
Highly Cited.
Beyond morphology: whole slide imaging, computer-aided detection, and other techniques.
Michael D Feldman.
Arch Pathol Lab Med, 2008 May 10; 132(5). PMID: 18466019
Regulation of glomerular volume in normal and partially nephrectomized rats.
P Cortes, X Zhao, B L Riser, R G Narins.
Am J Physiol, 1996 Feb 01; 270(2 Pt 2). PMID: 8779898
Treatment of hypertension in chronic kidney disease.
Robert D Toto.
Semin Nephrol, 2005 Nov 22; 25(6). PMID: 16298269
Review.
Digital pathology evaluation of complement C4d component deposition in the kidney allograft biopsies is a useful tool to improve reproducibility of the scoring.
Ernesta Brazdziute, Arvydas Laurinavicius.
Diagn Pathol, 2011 Apr 22; 6 Suppl 1. PMID: 21489200    Free PMC article.
Ovarian frozen section diagnosis: use of whole-slide imaging shows excellent correlation between virtual slide and original interpretations in a large series of cases.
Margaret A Fallon, David C Wilbur, Manju Prasad.
Arch Pathol Lab Med, 2010 Jul 01; 134(7). PMID: 20586631
Parathyroid hormone and phosphorus overload in uremia: impact on cardiovascular system.
Melani R Custódio, Marcia K Koike, +10 authors, Vanda Jorgetti.
Nephrol Dial Transplant, 2011 Aug 10; 27(4). PMID: 21825304
Digital pathology and image analysis for robust high-throughput quantitative assessment of Alzheimer disease neuropathologic changes.
Janna Hackett Neltner, Erin Lynn Abner, +4 authors, Peter T Nelson.
J Neuropathol Exp Neurol, 2012 Nov 14; 71(12). PMID: 23147505    Free PMC article.
Aberrant muscle antigen exposure in mice is sufficient to cause myositis in a Treg cell-deficient milieu.
Nicholas A Young, Rahul Sharma, +3 authors, Wael N Jarjour.
Arthritis Rheum, 2013 Sep 12; 65(12). PMID: 24022275    Free PMC article.
Assessment of hypoxia in the stroma of patient-derived pancreatic tumor xenografts.
Ines Lohse, Corey Lourenco, +3 authors, David W Hedley.
Cancers (Basel), 2014 Mar 01; 6(1). PMID: 24577243    Free PMC article.
Nicotinamide phosphoribosyltransferase inhibitor is a novel therapeutic candidate in murine models of inflammatory lung injury.
Liliana Moreno-Vinasco, Hector Quijada, +12 authors, Joe G N Garcia.
Am J Respir Cell Mol Biol, 2014 Mar 05; 51(2). PMID: 24588101    Free PMC article.
Quantification of myocardial fibrosis by digital image analysis and interactive stereology.
Dainius Daunoravicius, Justinas Besusparis, +8 authors, Arvydas Laurinavicius.
Diagn Pathol, 2014 Jun 11; 9. PMID: 24912374    Free PMC article.
Quantitative histopathological assessment of ocular surface squamous neoplasia using digital image analysis.
Rimvydas Ašoklis, Aistė Kadziauskienė, +2 authors, Arvydas Laurinavičius.
Oncol Lett, 2014 Sep 10; 8(4). PMID: 25202353    Free PMC article.
Soluble Serum αKlotho Is a Potential Predictive Marker of Disease Progression in Clear Cell Renal Cell Carcinoma.
Margherita Gigante, Giuseppe Lucarelli, +12 authors, Elena Ranieri.
Medicine (Baltimore), 2015 Nov 13; 94(45). PMID: 26559258    Free PMC article.
Renal Graft Fibrosis and Inflammation Quantification by an Automated Fourier-Transform Infrared Imaging Technique.
Vincent Vuiblet, Michael Fere, +3 authors, Philippe Rieu.
J Am Soc Nephrol, 2015 Dec 20; 27(8). PMID: 26683669    Free PMC article.
Multi-radial LBP Features as a Tool for Rapid Glomerular Detection and Assessment in Whole Slide Histopathology Images.
Olivier Simon, Rabi Yacoub, +2 authors, Pinaki Sarder.
Sci Rep, 2018 Feb 03; 8(1). PMID: 29391542    Free PMC article.
Deep Learning-Based Histopathologic Assessment of Kidney Tissue.
Meyke Hermsen, Thomas de Bel, +10 authors, Jeroen A W M van der Laak.
J Am Soc Nephrol, 2019 Sep 07; 30(10). PMID: 31488607    Free PMC article.
Deep Learning Global Glomerulosclerosis in Transplant Kidney Frozen Sections.
Jon N Marsh, Matthew K Matlock, +4 authors, S Joshua Swamidass.
IEEE Trans Med Imaging, 2018 Jul 12; 37(12). PMID: 29994669    Free PMC article.
Enhancing the Value of Histopathological Assessment of Allograft Biopsy Monitoring.
Michelle A Wood-Trageser, Andrew J Lesniak, Anthony J Demetris.
Transplantation, 2019 Feb 16; 103(7). PMID: 30768568    Free PMC article.
Review.
Prognostic value of B7-H1, B7-H3 and the stage, size, grade and necrosis (SSIGN) score in metastatic clear cell renal cell carcinoma.
Johannes Mischinger, Eleonore Fröhlich, +9 authors, Richard Zigeuner.
Cent European J Urol, 2019 Apr 24; 72(1). PMID: 31011436    Free PMC article.
Pathological manifestation of autoimmune myocarditis is detected prior to glomerulonephritis in a murine model of lupus nephritis.
Nicholas A Young, Kyle Jablonski, +10 authors, Wael N Jarjour.
Lupus, 2020 Oct 14; 29(13). PMID: 33045900    Free PMC article.
Digital Imaging Analysis Reveals Reduced Alveolar α-Smooth Muscle Actin Expression in Severe Asthma.
Jacob A Jerome, Sally E Wenzel, Humberto E Trejo Bittar.
Appl Immunohistochem Mol Morphol, 2021 Mar 13; 29(7). PMID: 33710120    Free PMC article.