Journal Article
. 2013 Sep; 30(6):2795-803.
doi: 10.3892/or.2013.2737.

Correlation and prognostic value of osteopontin and Bcl-2 in hepatocellular carcinoma patients after curative resection

Biao Deng 1 Xiao-Fei Zhang  Xu-Chao Zhu  Hua Huang  Hu-Liang Jia  Qing-Hai Ye  Qiong-Zhu Dong  Lun-Xiu Qin  
Affiliations
  • PMID: 24065086
  •     12 citations

Abstract

Osteopontin (OPN) may facilitate tumorigenesis and metastasis through prevention of tumor cells from apoptosis. Although previous studies have suggested involvement of enhanced Bcl-2 protein family expression, the role of OPN together with Bcl-2 in hepatocellular carcinoma (HCC) remains unknown. In this study, we used western blotting to detect the OPN and Bcl-2 expression levels in cell lines with different OPN backgrounds and HCC tissues, and tumor tissue microarrays to examine OPN and Bcl-2 expression levels in 454 HCC cases. The Kaplan-Meier method and log-rank test were applied to investigate the predictive values of OPN and Bcl-2 in HCC patients. In vitro assays indicated that OPN expression increased concordantly with increasing metastatic potential in MHCC97-H, MHCC97-L, HepG2 and SMMC-7721 cell lines by western blotting, whereas Bcl-2 expression declined. In addition, Bcl-2 was highly upregulated in OPN knockdown MHCC97-H cell lines. Furthermore, in HCC tissues, it was confirmed that OPN levels were also significantly higher in recurrent tumor tissues compared to non-recurrent tissues by western blotting (p<0.001), whereas the contrary occurred in Bcl-2 (p=0.046). Using immunohistochemistry analysis, patients with higher OPN levels had significantly shorter median survival time and recurrence time compared to the lower ones, although the opposite occurred in Bcl-2 levels. Of note, when OPN and Bcl-2 were combined, we found that the co-index of OPN/Bcl-2 was an independent prognostic factor for both overall survival (p<0.001) and time to recurrence (p<0.001). Our findings demonstrate that OPN/Bcl-2 expression is a promising independent predictor of recurrence and survival in HCC. Additionally, Bcl-2 levels may be regulated by OPN in the HCC microenvironment.

Role of osteopontin in the pathophysiology of cancer.
Lalita A Shevde, Rajeev S Samant.
Matrix Biol, 2014 Mar 25; 37. PMID: 24657887    Free PMC article.
Highly Cited. Review.
Can combination of osteopontin and peritumor-infiltrating macrophages be a prognostic marker of early-stage hepatocellular carcinoma?
Yoo Jin Lee, Byoung Kuk Jang.
Hepatobiliary Surg Nutr, 2014 May 09; 3(2). PMID: 24812596    Free PMC article.
Management of hepatocellular carcinoma: Predictive value of immunohistochemical markers for postoperative survival.
Zhao-Shan Niu, Xiao-Jun Niu, Mei Wang.
World J Hepatol, 2015 Jan 28; 7(1). PMID: 25624992    Free PMC article.
Review.
Inhibition of Vav3 gene can promote apoptosis of human gastric cancer cell line MGC803 by regulating ERK pathway.
Bi-Bo Tan, Ming-Ming Zhang, +4 authors, Dong Wang.
Tumour Biol, 2015 Dec 24; 37(6). PMID: 26695150
Silencing of ZNF139-siRNA induces apoptosis in human gastric cancer cell line BGC823.
Liqiao Fan, Bibo Tan, +4 authors, Zhidong Zhang.
Int J Clin Exp Pathol, 2016 Jan 02; 8(10). PMID: 26722429    Free PMC article.
Osteopontin promotes epithelial-mesenchymal transition of hepatocellular carcinoma through regulating vimentin.
Qiongzhu Dong, Xuchao Zhu, +16 authors, Lunxiu Qin.
Oncotarget, 2016 Jan 30; 7(11). PMID: 26824421    Free PMC article.
Bcl-2 expression is a poor predictor for hepatocellular carcinoma prognosis of andropause-age patients.
Xiao-Fei Zhang, Xin Yang, +14 authors, Lun-Xiu Qin.
Cancer Biol Med, 2017 Feb 06; 13(4). PMID: 28154777    Free PMC article.
SIRT1 overexpression protects non-small cell lung cancer cells against osteopontin-induced epithelial-mesenchymal transition by suppressing NF-κB signaling.
Xuejiao Li, Zhongxiu Jiang, Xiangmin Li, Xiaoye Zhang.
Onco Targets Ther, 2018 Mar 15; 11. PMID: 29535539    Free PMC article.
Prognostic value of osteopontin in patients with hepatocellular carcinoma: A systematic review and meta-analysis.
Tingting Sun, Peng Li, +2 authors, Guoqiang Li.
Medicine (Baltimore), 2018 Nov 10; 97(43). PMID: 30412113    Free PMC article.
Systematic Review.
Circulating osteopontin per tumor volume as a prognostic biomarker for resectable intrahepatic cholangiocarcinoma.
Kai-Qian Zhou, Wei-Feng Liu, +10 authors, Zheng Wang.
Hepatobiliary Surg Nutr, 2020 Jan 14; 8(6). PMID: 31929985    Free PMC article.
Osteopontin expression is associated with progression and adverse prognosis in patients with resectable gastrointestinal stromal tumor.
Jinhai Wang, Xiaobin Cheng, Sen Lu, Weina Fan.
Int J Clin Exp Pathol, 2020 Jan 15; 12(4). PMID: 31933953    Free PMC article.
Correlationship between Ki67, VEGF, and p53 and Hepatocellular Carcinoma Recurrence in Liver Transplant Patients.
Xia Zhang, Zhixian Wu, +7 authors, Kun Zhang.
Biomed Res Int, 2021 May 07; 2021. PMID: 33954191    Free PMC article.