Journal Article
. 2015 Feb; 13(1):68-73.
doi: 10.5217/ir.2015.13.1.68.

Circulating ghrelin levels and obestatin/ghrelin ratio as a marker of activity in ulcerative colitis

Ja Young Jung 1 Ji Bong Jeong 1 Ji Won Kim 1 Su Hwan Kim 1 Seong-Joon Koh 1 Byeong Gwan Kim 1 Kook Lae Lee 1 
Affiliations
  • PMID: 25691845
  •     24 References
  •     9 citations

Abstract

Background/Aims: Ghrelin levels are known to increase in patients with ulcerative colitis (UC), but serum obestatin levels in UC patients are not well elucidated. The aim of this study was to examine the relationship between serum ghrelin and obestatin levels and disease activity in UC patients.

Methods: The serum ghrelin and obestatin levels were measured in 21 UC patients (12 with active disease and 9 in remission) using enzyme-linked immunosorbent assay. The relationship between the circulating levels of these 2 hormones and disease activity was analyzed. The colonic mucosal mRNA expression of ghrelin and obestatin was measured by quantitative reverse transcription polymerase chain reaction.

Results: The mean serum ghrelin values were significantly higher in patients with active disease than in patients with remission (1370.6±404.3 vs. 783.5±235.3 pg/mL, P=0.001). Colonic mucosal mRNA expression of ghrelin was also significantly higher in patients with active disease than in patients in remission (0.805±0.214 vs. 0.481±0.356, P=0.018). However, the mean serum obestatin levels and colonic mucosal mRNA expression of obestatin were not significantly different between both groups. The circulating obestatin/ghrelin ratio was significantly lower in patients with active UC than in patients in remission (0.32±0.08 vs. 0.58±0.20, P=0.001).

Conclusions: The serum ghrelin levels and the obestatin/ghrelin ratio were related to the activity of UC, but serum obestatin was not related to activity of UC. The ghrelin levels and the obestatin/ghrelin ratio could serve as activity markers in patients with UC.

Keywords: Colitis, ulcerative; Disease activity; Ghrelin; Obestatin; Ratio.

Obestatin, a peptide encoded by the ghrelin gene, opposes ghrelin's effects on food intake.
Jian V Zhang, Pei-Gen Ren, +4 authors, Aaron J W Hsueh.
Science, 2005 Nov 15; 310(5750). PMID: 16284174
Highly Cited.
Ghrelin induces adiposity in rodents.
M Tschöp, D L Smiley, M L Heiman.
Nature, 2000 Nov 01; 407(6806). PMID: 11057670
Highly Cited.
Use of ghrelin as a treatment for inflammatory bowel disease: mechanistic considerations.
Mark D Deboer.
Int J Pept, 2011 Aug 17; 2011. PMID: 21845198    Free PMC article.
Leptin, adiponectin, resistin, and ghrelin--implications for inflammatory bowel disease.
Konstantinos Karmiris, Ioannis E Koutroubakis, Elias A Kouroumalis.
Mol Nutr Food Res, 2008 Apr 03; 52(8). PMID: 18383234
Review.
Endogenous and exogenous ghrelin enhance the colonic and gastric manifestations of dextran sodium sulphate-induced colitis in mice.
B De Smet, T Thijs, +6 authors, I Depoortere.
Neurogastroenterol Motil, 2008 Oct 01; 21(1). PMID: 18823291
Anti-inflammatory effect of the ghrelin agonist growth hormone-releasing peptide-2 (GHRP-2) in arthritic rats.
Miriam Granado, Teresa Priego, +2 authors, Asunción López-Calderón.
Am J Physiol Endocrinol Metab, 2004 Oct 28; 288(3). PMID: 15507538
Adipocytokines: mediators linking adipose tissue, inflammation and immunity.
Herbert Tilg, Alexander R Moschen.
Nat Rev Immunol, 2006 Sep 26; 6(10). PMID: 16998510
Highly Cited. Review.
Emergence of ghrelin as a treatment for cachexia syndromes.
Mark Daniel DeBoer.
Nutrition, 2008 Aug 30; 24(9). PMID: 18725076
Review.
Circulating ghrelin levels in celiac patients.
Maddalena Peracchi, Dario Conte, +5 authors, Maria Teresa Bardella.
Am J Gastroenterol, 2003 Nov 26; 98(11). PMID: 14638351
Circulating levels of leptin, adiponectin, resistin, and ghrelin in inflammatory bowel disease.
Konstantinos Karmiris, Ioannis E Koutroubakis, +3 authors, Elias A Kouroumalis.
Inflamm Bowel Dis, 2006 Jan 25; 12(2). PMID: 16432373
Review article: nutrition and adult inflammatory bowel disease.
J Goh, C A O'Morain.
Aliment Pharmacol Ther, 2003 Feb 04; 17(3). PMID: 12562443
Review.
The effect of infliximab on patients with ulcerative colitis in Korea.
Hyun Il Seo, Dong Il Park, +5 authors, Jeong Eun Shin.
Intest Res, 2014 Oct 29; 12(3). PMID: 25349595    Free PMC article.
Reduced plasma ghrelin concentration in celiac disease after gluten-free diet treatment.
Esmeralda Capristo, Sara Farnetti, +4 authors, Giovanni Gasbarrini.
Scand J Gastroenterol, 2005 Jul 21; 40(4). PMID: 16028437
Obestatin/ghrelin ratio: a new activity index in inflammatory bowel diseases.
Efstratios Alexandridis, Athanasios Zisimopoulos, +3 authors, Georgios Kouklakis.
Inflamm Bowel Dis, 2009 May 02; 15(10). PMID: 19408254
Phenotypical and functional study of ghrelin and its receptor in the pathogenesis of Crohn's disease.
Shuhei Hosomi, Nobuhide Oshitani, +9 authors, Tetsuo Arakawa.
Inflamm Bowel Dis, 2008 Apr 22; 14(9). PMID: 18425803
New concepts in the pathophysiology of inflammatory bowel disease.
Giorgos Bamias, Mark R Nyce, +3 authors, American Physiological Society.
Ann Intern Med, 2005 Dec 21; 143(12). PMID: 16365470
Review.
Production of adiponectin, an anti-inflammatory protein, in mesenteric adipose tissue in Crohn's disease.
K Yamamoto, T Kiyohara, +11 authors, Y Shinomura.
Gut, 2005 May 13; 54(6). PMID: 15888786    Free PMC article.
Resistin: another rising biomarker in inflammatory bowel disease?
Konstantinos Karmiris, Ioannis E Koutroubakis.
Eur J Gastroenterol Hepatol, 2007 Nov 14; 19(12). PMID: 17998823
Review.
Ghrelin inhibits leptin- and activation-induced proinflammatory cytokine expression by human monocytes and T cells.
Vishwa Deep Dixit, Eric M Schaffer, +5 authors, Dennis D Taub.
J Clin Invest, 2004 Jul 03; 114(1). PMID: 15232612    Free PMC article.
Highly Cited.
A role for ghrelin in the central regulation of feeding.
M Nakazato, N Murakami, +4 authors, S Matsukura.
Nature, 2001 Feb 24; 409(6817). PMID: 11196643
Highly Cited.
Circulating ghrelin level is increased in coeliac disease as in functional dyspepsia and reverts to normal during gluten-free diet.
A Lanzini, P Magni, +8 authors, C Ricci.
Aliment Pharmacol Ther, 2006 Apr 01; 23(7). PMID: 16573793
Review article: defining remission in ulcerative colitis.
S P L Travis, P D R Higgins, +9 authors, G D'Haens.
Aliment Pharmacol Ther, 2011 May 28; 34(2). PMID: 21615435
Review.
Helicobacter pylori infection modifies gastric and plasma ghrelin dynamics in Mongolian gerbils.
H Suzuki, T Masaoka, +5 authors, H Ishii.
Gut, 2004 Jan 16; 53(2). PMID: 14724148    Free PMC article.
Therapeutic action of ghrelin in a mouse model of colitis.
Elena Gonzalez-Rey, Alejo Chorny, Mario Delgado.
Gastroenterology, 2006 May 16; 130(6). PMID: 16697735
Obestatin Accelerates the Healing of Acetic Acid-Induced Colitis in Rats.
Aleksandra Matuszyk, Piotr Ceranowicz, +7 authors, Artur Dembiński.
Oxid Med Cell Longev, 2016 Jan 23; 2016. PMID: 26798415    Free PMC article.
Obestatin as a key regulator of metabolism and cardiovascular function with emerging therapeutic potential for diabetes.
Elaine Cowan, Kerry J Burch, Brian D Green, David J Grieve.
Br J Pharmacol, 2016 Apr 26; 173(14). PMID: 27111465    Free PMC article.
Review.
Plasma ghrelin level and plasma ghrelin/obestatin ratio are related to intestinal metaplasia in elderly patients with functional dyspepsia.
Su Hwan Kim, Ji Won Kim, +3 authors, Kook Lae Lee.
PLoS One, 2017 Apr 19; 12(4). PMID: 28419119    Free PMC article.
Plasma ghrelin and leptin in patients with inflammatory bowel disease and its association with nutritional status.
Firas A Ghomraoui, Sami T Alotaibi, +9 authors, Mohammad A Alzoghaibi.
Saudi J Gastroenterol, 2017 Jun 15; 23(3). PMID: 28611344    Free PMC article.
NF-kappa B activation correlates with disease phenotype in Crohn's disease.
Yoo Min Han, Jaemoon Koh, +6 authors, Joo Sung Kim.
PLoS One, 2017 Jul 29; 12(7). PMID: 28753650    Free PMC article.
TNF-α inhibits SCF, ghrelin, and substance P expressions through the NF-κB pathway activation in interstitial cells of Cajal.
Keyu Ren, Chunming Yong, +3 authors, Jin Wang.
Braz J Med Biol Res, 2018 Apr 26; 51(6). PMID: 29694505    Free PMC article.
Treatment with Obestatin-A Ghrelin Gene-Encoded Peptide-Reduces the Severity of Experimental Colitis Evoked by Trinitrobenzene Sulfonic Acid.
Katarzyna Konarska, Jakub Cieszkowski, +9 authors, Artur Dembiński.
Int J Mol Sci, 2018 Jun 06; 19(6). PMID: 29865176    Free PMC article.
Pretreatment with obestatin inhibits the development of acetic acid-induced colitis in rats.
Aleksandra Matuszyk, Piotr Ceranowicz, +7 authors, Artur Dembiński.
Arch Med Sci, 2018 Jul 14; 14(4). PMID: 30002711    Free PMC article.
L-Glutamine and Physical Exercise Prevent Intestinal Inflammation and Oxidative Stress Without Improving Gastric Dysmotility in Rats with Ulcerative Colitis.
Raisa de Oliveira Santos, Geovane da Silva Cardoso, +12 authors, Moisés Tolentino.
Inflammation, 2020 Nov 01; 44(2). PMID: 33128666