Journal Article
. 2015 Aug;10(8).
doi: 10.1371/journal.pone.0133562.

Prognostic Fifteen-Gene Signature for Early Stage Pancreatic Ductal Adenocarcinoma

Dung-Tsa Chen 1 Ashley H Davis-Yadley 2 Po-Yu Huang 3 Kazim Husain 2 Barbara A Centeno 4 Jennifer Permuth-Wey 5 Jose M Pimiento 2 Mokenge Malafa 2 
Affiliations
  • PMID: 26247463
  •     46 References
  •     27 citations

Abstract

The outcomes of patients treated with surgery for early stage pancreatic ductal adenocarcinoma (PDAC) are variable with median survival ranging from 6 months to more than 5 years. This challenge underscores an unmet need for developing personalized medicine strategies to refine the current treatment decision-making process. To derive a prognostic gene signature for patients with early stage PDAC, a PDAC cohort from Moffitt Cancer Center (n = 63) was used with overall survival (OS) as the primary endpoint. This was further evaluated using an independent microarray cohort dataset (Stratford et al: n = 102). Technical validation was performed by NanoString platform. A prognostic 15-gene signature was developed and showed a statistically significant association with OS in the Moffitt cohort (hazard ratio [HR] = 3.26; p<0.001) and Stratford et al cohort (HR = 2.07; p = 0.02), and was independent of other prognostic variables. Moreover, integration of the signature with the TNM staging system improved risk prediction (p<0.01 in both cohorts). In addition, NanoString validation showed that the signature was robust with a high degree of reproducibility and the association with OS remained significant in the two cohorts. The gene signature could be a potential prognostic tool to allow risk-adapted stratification of PDAC patients into personalized treatment protocols; possibly improving the currently poor clinical outcomes of these patients.

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