Journal Article
. 2016 Apr; 7:10.
doi: 10.4103/2153-3539.177687.

Comparison of the diagnostic utility of digital pathology systems for telemicrobiology

Daniel D Rhoads 1 Nadia F Habib-Bein 2 Rahman S Hariri 3 Douglas J Hartman 3 Sara E Monaco 3 Andrew Lesniak 3 Jon Duboy 3 Mohamed El-Sayed Salama 4 Liron Pantanowitz 3 
Affiliations
  • PMID: 27076988
  •     7 References
  •     4 citations

Abstract

Introduction: Telemicrobiology is a growing component of clinical microbiology informatics. However, few studies have been performed to assess the diagnostic utility of telemicroscopy systems in evaluating infectious agents.

Objective: Evaluate multiple contemporary digital pathology platforms for use in diagnostic telemicrobiology.

Materials And Methods: A mix of thirty cases that included viral, bacterial, fungal, and parasitological findings were evaluated by four experts using ×40 whole slide imaging (WSI) scans, ×83 oil-immersion WSI scans, ×100 oil-immersion WSI scans, digital photomicrographs, and glass slides.

Results: The ×83 WSI, ×100 WSI, and photomicrograph interpretations were not significantly different in quality and accuracy when compared to glass slide interpretations. The ×40 WSI interpretations were of lower quality and were more likely to be incorrect when compared to glass slide interpretations.

Conclusions: In this study, high magnification, oil-immersion digital pathology platforms are better suited to support telemicrobiology applications and yield interpretations on par with glass slide evaluations.

Keywords: Digital pathology; infectious diseases; microbiology; telemicrobiology; telemicroscopy; whole slide imaging.

Telemicrobiology: feasibility study.
W J McLaughlin, R B Schifman, +4 authors, R S Weinstein.
Telemed J, 1998 May 23; 4(1). PMID: 9599069
Concordance between whole-slide imaging and light microscopy for routine surgical pathology.
W Scott Campbell, Subodh M Lele, +3 authors, Steven H Hinrichs.
Hum Pathol, 2012 May 18; 43(10). PMID: 22591912
Virtual 3D microscopy using multiplane whole slide images in diagnostic pathology.
Thomas Kalinski, Ralf Zwönitzer, +5 authors, Albert Roessner.
Am J Clin Pathol, 2008 Jul 17; 130(2). PMID: 18628096
Clinical microbiology informatics.
Daniel D Rhoads, Vitali Sintchenko, Carol A Rauch, Liron Pantanowitz.
Clin Microbiol Rev, 2014 Oct 04; 27(4). PMID: 25278581    Free PMC article.
Review.
Web-based oil immersion whole slide imaging increases efficiency and clinical team satisfaction in hematopathology tumor board.
Zhongchuan Will Chen, Jessica Kohan, +2 authors, Mohamed E Salama.
J Pathol Inform, 2014 Nov 08; 5(1). PMID: 25379347    Free PMC article.
Whole Slide Imaging of Pap Cell Block Preparations versus Liquid-Based Thin-Layer Cervical Cytology: A Comparative Study Evaluating the Detection of Organisms and Nonneoplastic Findings.
Ossama Tawfik, Marilyn Davis, +3 authors, Fang Fan.
Acta Cytol, 2014 Jul 19; 58(4). PMID: 25033897
Telemicrobiology: a novel telemedicine capability for mission support in the field of infectious medicine.
Patrick Scheid, David M Lam, Alois Thömmes, Lothar Zöller.
Telemed J E Health, 2007 May 11; 13(2). PMID: 17489697
Validation of a digital pathology system including remote review during the COVID-19 pandemic.
Matthew G Hanna, Victor E Reuter, +21 authors, Meera Hameed.
Mod Pathol, 2020 Jun 24; 33(11). PMID: 32572154    Free PMC article.
The Use of Screencasts with Embedded Whole-Slide Scans and Hyperlinks to Teach Anatomic Pathology in a Supervised Digital Environment.
Mary Wong, Joseph Frye, Stacey Kim, Alberto M Marchevsky.
J Pathol Inform, 2019 Jan 05; 9. PMID: 30607306    Free PMC article.
Remote Reporting from Home for Primary Diagnosis in Surgical Pathology: A Tertiary Oncology Center Experience during the COVID-19 Pandemic.
Vidya Rao, Rajiv Kumar, +17 authors, Sangeeta Desai.
J Pathol Inform, 2021 May 21; 12. PMID: 34012707    Free PMC article.
Experience Reviewing Digital Pap Tests using a Gallery of Images.
Liron Pantanowitz, Sarah Harrington.
J Pathol Inform, 2021 May 21; 12. PMID: 34012711    Free PMC article.