Journal Article
. 2017 Jul; 2(14):.
doi: 10.1172/jci.insight.93652.

Multiparametric immune profiling in HPV- oral squamous cell cancer

Zipei Feng 1 Daniel Bethmann 1 Matthias Kappler 2 Carmen Ballesteros-Merino 1 Alexander Eckert 2 R Bryan Bell 1 Allen Cheng 3 Tuan Bui 3 Rom Leidner 1 Walter J Urba 1 Kent Johnson 4 Clifford Hoyt 4 Carlo B Bifulco 1 Juergen Bukur 5 Claudia Wickenhauser 6 Barbara Seliger 5 Bernard A Fox 1 
Affiliations
  • PMID: 28724788
  •     53 References
  •     59 citations

Abstract

Evaluation of T lymphocyte frequency provides prognostic information for patients with oral squamous cell cancer (OSCC). However, the effect of simultaneously evaluating T cell frequency and assessing suppressive elements and defects in antigen-processing machinery (APM) has not been clarified. Simultaneous characterization of CD3+, CD8+, FoxP3+, CD163+, and PD-L1+ cells using multispectral imaging was performed on sections from 119 patients with HPV- OSCC. Expression of β2-microglobulin, MHC class I heavy chain, and large multifunctional peptidase 10 was quantified, and all data were correlated with patient outcome. We found that, consistent with previous reports, high numbers of CD8+ T cells at the invasive margin correlated significantly with prolonged overall survival (OS), while the number of FoxP3+ or PD-L1+ cells did not. Compiling the number of FoxP3+ or PD-L1+ cells within 30 μm of CD8+ T cells identified a significant association with a high number of suppressive elements close to CD8+ T cells and reduced OS. Integrating this information into a cumulative suppression index (CSI) increased correlation with OS. Incorporating tumor expression levels of APM components with CSI further improved prognostic power. This multiparametric immune profiling may be useful for stratifying patients with OSCC for clinical trials.

Keywords: Immunology; Oncology.

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Maximilian Kießler, Ioana Plesca, +16 authors, Marc Schmitz.
J Immunother Cancer, 2021 Mar 26; 9(3). PMID: 33762320    Free PMC article.
Next-Generation Digital Histopathology of the Tumor Microenvironment.
Felicitas Mungenast, Achala Fernando, +4 authors, Rupert C Ecker.
Genes (Basel), 2021 May 01; 12(4). PMID: 33917241    Free PMC article.
Review.
Immune landscape and subtypes in primary resectable oral squamous cell carcinoma: prognostic significance and predictive of therapeutic response.
Pengfei Diao, Yue Jiang, +17 authors, Jie Cheng.
J Immunother Cancer, 2021 Jun 17; 9(6). PMID: 34130988    Free PMC article.
Explant Modeling of the Immune Environment of Head and Neck Cancer.
Shay Sharon, Thomas Duhen, +9 authors, Michael Gough.
Front Oncol, 2021 Jul 06; 11. PMID: 34221953    Free PMC article.
Multi-institutional TSA-amplified Multiplexed Immunofluorescence Reproducibility Evaluation (MITRE) Study.
Janis M Taube, Kristin Roman, +13 authors, Cliff Hoyt.
J Immunother Cancer, 2021 Jul 17; 9(7). PMID: 34266881    Free PMC article.
CD30+OX40+ Treg is associated with improved overall survival in colorectal cancer.
Jian Hang Lam, Michelle Hong, +10 authors, Tong Seng Lim.
Cancer Immunol Immunother, 2021 Feb 03; 70(8). PMID: 33527196    Free PMC article.
High PD-L1 Expression on Tumor Cells Indicates Worse Overall Survival in Advanced Oral Squamous Cell Carcinomas of the Tongue and the Floor of the Mouth but Not in Other Oral Compartments.
Łukasz Jan Adamski, Anna Starzyńska, +7 authors, Rafał Pęksa.
Biomedicines, 2021 Sep 29; 9(9). PMID: 34572318    Free PMC article.
Human tissue cultures of lung cancer predict patient susceptibility to immune-checkpoint inhibition.
David Junk, Sebastian Krämer, +10 authors, Sonja Kallendrusch.
Cell Death Discov, 2021 Sep 27; 7(1). PMID: 34564709    Free PMC article.
A nomogram-based immunoprofile predicts clinical outcomes for stage II and III human colorectal cancer.
Lingxiong Wang, Nijia Chang, +7 authors, Shunchang Jiao.
Mol Clin Oncol, 2021 Oct 30; 15(6). PMID: 34712487    Free PMC article.
Spatial UMAP and Image Cytometry for Topographic Immuno-oncology Biomarker Discovery.
Nicolas A Giraldo, Sneha Berry, +16 authors, Janis M Taube.
Cancer Immunol Res, 2021 Aug 27; 9(11). PMID: 34433588    Free PMC article.