Journal Article
. 2017 Sep; 7(3):247-250.
doi: 10.3892/br.2017.950.

Comparison between digital and optical microscopy: Analysis in a mouse gut inflammation model

Airton Pereira E Silva 1 Sylvia Maria Nicolau Campos 1 Isabelle Mazza Guimarães 1 Gerlinde Agate Platais Brasil Teixeira 1 
Affiliations
  • PMID: 28894572
  •     29 References
  •     3 citations

Abstract

Virtual microscopy is currently widely used for various purposes, such as teaching, archiving, collaborations and research. Although the cost of this technique has reduced, it continues to be expensive for the majority of laboratories in developing countries. The Graduate Program in Pathology at the Federal Fluminense University (Niterói, Brazil) has acquired equipment for virtual microscopy. However, this novel method faced prejudice, as students and technicians were skeptical about its reliability. Thus, the aim of the current study was to evaluate whether virtual microscopy is a reliable method of analysis for our research. Thus, a mouse gut inflammation model developed by our research group was used in the present study. Analysis was performed using optical microscopy and digital imaging using the APERIO scanning system and the ImageScope® software. Intestinal epithelial cells (IECs), intra epithelial leucocytes (IEL), and villi number and area were evaluated. No significant differences were observed in villi number, IEC and IEL; however, the villi area was significantly smaller when measured using the computer. Thus, the present study indicates that virtual microscopy is a trustworthy method for research purposes.

Keywords: digital; gut inflammation; mouse; optical microscopy; virtual microscopy; whole slide image.

A microscope for use in dentistry.
H Apotheker, G J Jako.
J Microsurg, 1981 Jan 01; 3(1). PMID: 7341737
Food allergy/hypersensitivity: antigenicity or timing?
Patrícia Olaya Paschoal, Sylvia M N Campos, +5 authors, Gerlinde Teixeira.
Immunobiology, 2009 Mar 31; 214(4). PMID: 19327544
The correction of electron lens aberrations.
P W Hawkes.
Ultramicroscopy, 2015 May 31; 156. PMID: 26025209
Review.
Digital image tiles: a method for the processing of large sections.
D A Silage, J Gil.
J Microsc, 1985 May 01; 138(Pt 2). PMID: 3894672
Non-distorted assemblage of the digital images of adjacent fields in histological sections.
D Westerkamp, T Gahm.
Anal Cell Pathol, 1993 Jul 01; 5(4). PMID: 8363984
Quantitating the cell: turning images into numbers with ImageJ.
Ellen T Arena, Curtis T Rueden, +3 authors, Kevin W Eliceiri.
Wiley Interdiscip Rev Dev Biol, 2016 Dec 03; 6(2). PMID: 27911038
Review.
Utility of whole slide imaging and virtual microscopy in prostate pathology.
Philippe Camparo, Lars Egevad, +12 authors, Murali Varma.
APMIS, 2012 Mar 21; 120(4). PMID: 22429212
Review.
Summary of 2(nd) Nordic symposium on digital pathology.
Claes Lundström, Sten Thorstenson, +2 authors, Darren Treanor.
J Pathol Inform, 2015 Mar 17; 6. PMID: 25774316    Free PMC article.
Objectifying the conjunctival provocation test: photography-based rating and digital analysis.
S Dogan, A Astvatsatourov, +4 authors, R Mösges.
Int Arch Allergy Immunol, 2013 Nov 20; 163(1). PMID: 24248100
Virtual microscopy in pathology education.
Fred R Dee.
Hum Pathol, 2009 Jun 23; 40(8). PMID: 19540551
The histopathology of coeliac disease: time for a standardized report scheme for pathologists.
G Oberhuber, G Granditsch, H Vogelsang.
Eur J Gastroenterol Hepatol, 1999 Oct 19; 11(10). PMID: 10524652
Highly Cited. Review.
Whole slide imaging: uses and limitations for surgical pathology and teaching.
B F Boyce.
Biotech Histochem, 2015 Apr 23; 90(5). PMID: 25901738
Review.
Applications and challenges of digital pathology and whole slide imaging.
C Higgins.
Biotech Histochem, 2015 May 16; 90(5). PMID: 25978139
Review.
The surface area of the intestinal mucosa in the rat and in the cat.
H O Wood.
J Anat, 1944 Apr 01; 78(Pt 3). PMID: 17104943    Free PMC article.
The use of digital images in pathology.
P N Furness.
J Pathol, 1998 Jan 10; 183(3). PMID: 9422979
Review.
Maternal immunomodulation of the offspring's immunological system.
Sylvia M N Campos, Vivian L de Oliveira, +4 authors, Gerlinde Agate Platais Brasil Teixeira.
Immunobiology, 2014 Aug 12; 219(11). PMID: 25104403
Diet selection in immunologically manipulated mice.
Gerlinde Teixeira, Patrícia Olaya Paschoal, +4 authors, Alberto Nóbrega.
Immunobiology, 2008 Jan 22; 213(1). PMID: 18207023
Food allergy: immune mechanisms, diagnosis and immunotherapy.
Wong Yu, Deborah M Hussey Freeland, Kari C Nadeau.
Nat Rev Immunol, 2016 Nov 01; 16(12). PMID: 27795547    Free PMC article.
Highly Cited. Review.
Coeliac disease: an update for pathologists.
B C Dickson, C J Streutker, R Chetty.
J Clin Pathol, 2006 Oct 06; 59(10). PMID: 17021129    Free PMC article.
Review.
Image Montaging for Creating a Virtual Pathology Slide: An Innovative and Economical Tool to Obtain a Whole Slide Image.
Spoorthi Ravi Banavar, Prashanthi Chippagiri, +2 authors, Premalatha Bidadi Rajashekaraiah.
Anal Cell Pathol (Amst), 2016 Oct 18; 2016. PMID: 27747147    Free PMC article.
Automated image analysis in the study of lymphocyte subpopulation in eosinophilic oesophagitis.
Marcial García-Rojo, Joaquín Sánchez, +8 authors, Bartolomé López-Viedma.
Diagn Pathol, 2015 Jan 08; 9 Suppl 1. PMID: 25565117    Free PMC article.
The gradient of mucosal surface area in the small intestine of the rat.
R B FISHER, D S PARSONS.
J Anat, 1950 Jul 01; 84(3). PMID: 15436331    Free PMC article.
Highly Cited.
Review of the current state of whole slide imaging in pathology.
Liron Pantanowitz, Paul N Valenstein, +5 authors, Terence J Colgan.
J Pathol Inform, 2011 Sep 03; 2. PMID: 21886892    Free PMC article.
Highly Cited.
[Allergic rhinoconjunctivitis. Guidelines of the DGAI in association with the DDG].
Claus Bachert, Ulrich Borchard, +6 authors, Johannes Ring.
J Dtsch Dermatol Ges, 2006 Apr 22; 4(3). PMID: 16626325
Digital slides: present status of a tool for consultation, teaching, and quality control in pathology.
Rafael Rocha, José Vassallo, +2 authors, Helenice Gobbi.
Pathol Res Pract, 2009 Jun 09; 205(11). PMID: 19501988
Review.
A history of scanning electron microscopy developments: towards "wet-STEM" imaging.
A Bogner, P-H Jouneau, +2 authors, C Gauthier.
Micron, 2006 Sep 23; 38(4). PMID: 16990007
Virtual microscopy and digital pathology in training and education.
Peter W Hamilton, Yinhai Wang, Stephen J McCullough.
APMIS, 2012 Mar 21; 120(4). PMID: 22429213
Review.
"Slide less pathology": Fairy tale or reality?
M Indu, R Rathy, M P Binu.
J Oral Maxillofac Pathol, 2016 Sep 08; 20(2). PMID: 27601824    Free PMC article.
Review.
In vivo subcellular resolution optical imaging in the lung reveals early metastatic proliferation and motility.
David Entenberg, Carolina Rodriguez-Tirado, +3 authors, John Condeelis.
Intravital, 2016 Feb 09; 4(3). PMID: 26855844    Free PMC article.
A histomorphometric classification system for normal and inflamed mouse duodenum-Quali-quantitative approach.
Airton Pereira E Silva, João R A Soares, +4 authors, Gerlinde A P B Teixeira.
Int J Exp Pathol, 2018 Sep 04; 99(4). PMID: 30175413    Free PMC article.
Inhibition of Mixed Biofilms of Candida albicans and Methicillin-Resistant Staphylococcus aureus by Positively Charged Silver Nanoparticles and Functionalized Silicone Elastomers.
Humberto H Lara, Jose L Lopez-Ribot.
Pathogens, 2020 Oct 01; 9(10). PMID: 32992727    Free PMC article.
Comparison of the whole slide imaging and conventional light microscopy in the grading of oral epithelial dysplasia: a multi-institutional study.
P-B Diniz, M Sena-Filho, +4 authors, J Jorge.
Med Oral Patol Oral Cir Bucal, 2020 Nov 29; 26(1). PMID: 33247580    Free PMC article.