Journal Article
. 2017 Sep; 42(1):39-52.
doi: 10.1097/PAS.0000000000000948.

Whole Slide Imaging Versus Microscopy for Primary Diagnosis in Surgical Pathology: A Multicenter Blinded Randomized Noninferiority Study of 1992 Cases (Pivotal Study)

Sanjay Mukhopadhyay 1 Michael D Feldman 2 Esther Abels 3 Raheela Ashfaq 4 Senda Beltaifa 5 Nicolas G Cacciabeve 5 Helen P Cathro 6 Liang Cheng 7 Kumarasen Cooper 2 Glenn E Dickey 5 Ryan M Gill 8 Robert P Heaton 5 René Kerstens 3 Guy M Lindberg 4 Reenu K Malhotra 4 James W Mandell 6 Ellen D Manlucu 5 Anne M Mills 6 Stacey E Mills 6 Christopher A Moskaluk 6 Mischa Nelis 3 Deepa T Patil 1 Christopher G Przybycin 1 Jordan P Reynolds 1 Brian P Rubin 1 Mohammad H Saboorian 4 Mauricio Salicru 4 Mark A Samols 5 Charles D Sturgis 1 Kevin O Turner 4 Mark R Wick 6 Ji Y Yoon 4 Po Zhao 5 Clive R Taylor 9 
Affiliations
  • PMID: 28961557
  •     54 References
  •     58 citations

Abstract

Most prior studies of primary diagnosis in surgical pathology using whole slide imaging (WSI) versus microscopy have focused on specific organ systems or included relatively few cases. The objective of this study was to demonstrate that WSI is noninferior to microscopy for primary diagnosis in surgical pathology. A blinded randomized noninferiority study was conducted across the entire range of surgical pathology cases (biopsies and resections, including hematoxylin and eosin, immunohistochemistry, and special stains) from 4 institutions using the original sign-out diagnosis (baseline diagnosis) as the reference standard. Cases were scanned, converted to WSI and randomized. Sixteen pathologists interpreted cases by microscopy or WSI, followed by a wash-out period of ≥4 weeks, after which cases were read by the same observers using the other modality. Major discordances were identified by an adjudication panel, and the differences between major discordance rates for both microscopy (against the reference standard) and WSI (against the reference standard) were calculated. A total of 1992 cases were included, resulting in 15,925 reads. The major discordance rate with the reference standard diagnosis was 4.9% for WSI and 4.6% for microscopy. The difference between major discordance rates for microscopy and WSI was 0.4% (95% confidence interval, -0.30% to 1.01%). The difference in major discordance rates for WSI and microscopy was highest in endocrine pathology (1.8%), neoplastic kidney pathology (1.5%), urinary bladder pathology (1.3%), and gynecologic pathology (1.2%). Detailed analysis of these cases revealed no instances where interpretation by WSI was consistently inaccurate compared with microscopy for multiple observers. We conclude that WSI is noninferior to microscopy for primary diagnosis in surgical pathology, including biopsies and resections stained with hematoxylin and eosin, immunohistochemistry and special stains. This conclusion is valid across a wide variety of organ systems and specimen types.

Validation of whole slide imaging in the primary diagnosis of gynaecological pathology in a University Hospital.
Jaume Ordi, Paola Castillo, +4 authors, Jose Ramírez.
J Clin Pathol, 2014 Oct 31; 68(1). PMID: 25355520
Patient safety in anatomic pathology: measuring discrepancy frequencies and causes.
Stephen S Raab, Raouf E Nakhleh, Stephen G Ruby.
Arch Pathol Lab Med, 2005 Mar 30; 129(4). PMID: 15794667
iPathology cockpit diagnostic station: validation according to College of American Pathologists Pathology and Laboratory Quality Center recommendation at the Hospital Trust and University of Verona.
Matteo Brunelli, Serena Beccari, +8 authors, Albino Eccher.
Diagn Pathol, 2015 Jan 08; 9 Suppl 1. PMID: 25565219    Free PMC article.
Virtual telepathology in Egypt, applications of WSI in Cairo University.
Essam Ayad.
Diagn Pathol, 2011 Apr 22; 6 Suppl 1. PMID: 21489180    Free PMC article.
A comparison of cervical histopathology variability using whole slide digitized images versus glass slides: experience with a statewide registry.
Julia C Gage, Nancy Joste, +4 authors, Cosette M Wheeler.
Hum Pathol, 2013 Oct 01; 44(11). PMID: 24075599    Free PMC article.
Whole-slide imaging digital pathology as a platform for teleconsultation: a pilot study using paired subspecialist correlations.
David C Wilbur, Kalil Madi, +22 authors, Wolfgang Klietmann.
Arch Pathol Lab Med, 2009 Dec 08; 133(12). PMID: 19961250    Free PMC article.
Validation of diagnostic accuracy using digital slides in routine histopathology.
László Fónyad, Tibor Krenács, +7 authors, Béla Molnár.
Diagn Pathol, 2012 Apr 03; 7. PMID: 22463804    Free PMC article.
Intra-observer reproducibility of whole slide imaging for the primary diagnosis of breast needle biopsies.
Carolina Reyes, Offiong F Ikpatt, Mehrdad Nadji, Richard J Cote.
J Pathol Inform, 2014 Apr 18; 5(1). PMID: 24741464    Free PMC article.
From microscopy to whole slide digital images: a century and a half of image analysis.
Clive R Taylor.
Appl Immunohistochem Mol Morphol, 2011 Nov 18; 19(6). PMID: 22089486
Review.
Validation of whole slide imaging for primary diagnosis in surgical pathology.
Thomas W Bauer, Lynn Schoenfield, +3 authors, Walter H Henricks.
Arch Pathol Lab Med, 2013 Jan 18; 137(4). PMID: 23323732
Interinstitutional whole slide imaging teleconsultation service development: assessment using internal training and clinical consultation cases.
Nicholas C Jones, Rosalynn M Nazarian, +10 authors, David C Wilbur.
Arch Pathol Lab Med, 2014 Nov 22; 139(5). PMID: 25415180
Validating whole-slide imaging for consultation diagnoses in surgical pathology.
Thomas W Bauer, Renee J Slaw.
Arch Pathol Lab Med, 2014 May 21; 138(11). PMID: 24840034
High agreement between whole slide imaging and optical microscopy for assessment of HER2 expression in breast cancer: whole slide imaging for the assessment of HER2 expression.
Cristiana Nunes, Rafael Rocha, +4 authors, Helenice Gobbi.
Pathol Res Pract, 2014 Aug 06; 210(11). PMID: 25091257
Concordance between whole-slide imaging and light microscopy for routine surgical pathology.
W Scott Campbell, Subodh M Lele, +3 authors, Steven H Hinrichs.
Hum Pathol, 2012 May 18; 43(10). PMID: 22591912
Reading immunohistochemical slides on a computer monitor--a multisite performance study using 180 HER2-stained breast carcinomas.
Aziza Nassar, Cynthia Cohen, +6 authors, Momin T Siddiqui.
Appl Immunohistochem Mol Morphol, 2011 Apr 09; 19(3). PMID: 21475038
Evaluation of immunohistochemical staining using whole-slide imaging for HER2 scoring of breast cancer in comparison with real glass slides.
Yuzuru Kondo, Tatsuo Iijima, Masayuki Noguchi.
Pathol Int, 2012 Aug 29; 62(9). PMID: 22924845
Whole slide imaging for educational purposes.
Liron Pantanowitz, Janusz Szymas, Yukako Yagi, David Wilbur.
J Pathol Inform, 2013 Feb 02; 3. PMID: 23372987    Free PMC article.
Evaluation of whole slide image immunohistochemistry interpretation in challenging prostate needle biopsies.
Jeffrey L Fine, Dana M Grzybicki, +8 authors, Drazen M Jukic.
Hum Pathol, 2008 Feb 01; 39(4). PMID: 18234276
Reliability of whole slide images as a diagnostic modality for renal allograft biopsies.
Kuang-Yu Jen, Jean L Olson, +3 authors, Zoltan G Laszik.
Hum Pathol, 2012 Dec 04; 44(5). PMID: 23199528
Variability in diagnostic opinion among pathologists for single small atypical foci in prostate biopsies.
Theodorus H Van der Kwast, Andrew Evans, +15 authors, John Srigley.
Am J Surg Pathol, 2010 Jan 12; 34(2). PMID: 20061936
Use of whole slide imaging in surgical pathology quality assurance: design and pilot validation studies.
Jonhan Ho, Anil V Parwani, +3 authors, John R Gilbertson.
Hum Pathol, 2006 Apr 15; 37(3). PMID: 16613327
Concordance between digital pathology and light microscopy in general surgical pathology: a pilot study of 100 cases.
Joseph P Houghton, Aaron J Ervine, +5 authors, Peter W Hamilton.
J Clin Pathol, 2014 Sep 25; 67(12). PMID: 25248822
The College of American Pathologists guidelines for whole slide imaging validation are feasible for pediatric pathology: a pediatric pathology practice experience.
Michael A Arnold, Emily Chenever, +9 authors, Thomas Barr.
Pediatr Dev Pathol, 2014 Nov 12; 18(2). PMID: 25387255
Interobserver and intraobserver variation among experts in the diagnosis of thyroid follicular lesions with borderline nuclear features of papillary carcinoma.
Tarik M Elsheikh, Sylvia L Asa, +4 authors, Bruce M Wenig.
Am J Clin Pathol, 2008 Oct 16; 130(5). PMID: 18854266
Virtual 3D microscopy using multiplane whole slide images in diagnostic pathology.
Thomas Kalinski, Ralf Zwönitzer, +5 authors, Albert Roessner.
Am J Clin Pathol, 2008 Jul 17; 130(2). PMID: 18628096
Interobserver and intraobserver reproducibility in digital and routine microscopic assessment of prostate needle biopsies.
Paula A Rodriguez-Urrego, Angel M Cronin, +4 authors, Samson W Fine.
Hum Pathol, 2010 Oct 26; 42(1). PMID: 20970164
Using the Periscope Live Video-Streaming Application for Global Pathology Education: A Brief Introduction.
Maren Y Fuller, Sanjay Mukhopadhyay, Jerad M Gardner.
Arch Pathol Lab Med, 2016 Jul 22; 140(11). PMID: 27441785
Diagnostic Efficiency in Digital Pathology: A Comparison of Optical Versus Digital Assessment in 510 Surgical Pathology Cases.
Anne M Mills, Sarah E Gradecki, +5 authors, Helen P Cathro.
Am J Surg Pathol, 2017 Sep 07; 42(1). PMID: 28877052
A multisite performance study comparing the reading of immunohistochemical slides on a computer monitor with conventional manual microscopy for estrogen and progesterone receptor analysis.
Aziza Nassar, Cynthia Cohen, +9 authors, Momin T Siddiqui.
Am J Clin Pathol, 2011 Feb 26; 135(3). PMID: 21350103
Validation of a whole slide imaging system for primary diagnosis in surgical pathology: A community hospital experience.
Thomas P Buck, Rebecca Dilorio, Lauren Havrilla, Dennis G O'Neill.
J Pathol Inform, 2014 Dec 24; 5(1). PMID: 25535591    Free PMC article.
The potential impact of reproducibility of Gleason grading in men with early stage prostate cancer managed by active surveillance: a multi-institutional study.
Jesse K McKenney, Jeff Simko, +12 authors, Canary/Early Detection Research Network Prostate Active Surveillance Study Investigators.
J Urol, 2011 Jun 18; 186(2). PMID: 21679996
Digital slide viewing for primary reporting in gastrointestinal pathology: a validation study.
Maurice B Loughrey, Paul J Kelly, +5 authors, Peter W Hamilton.
Virchows Arch, 2015 May 20; 467(2). PMID: 25982098
Validation of digital pathology imaging for primary histopathological diagnosis.
David R J Snead, Yee-Wah Tsang, +16 authors, Ian A Cree.
Histopathology, 2015 Sep 27; 68(7). PMID: 26409165
Primary histologic diagnosis using automated whole slide imaging: a validation study.
John R Gilbertson, Jonhan Ho, +3 authors, Anil V Parwani.
BMC Clin Pathol, 2006 Apr 29; 6. PMID: 16643664    Free PMC article.
Overview of contemporary guidelines in digital pathology: what is available in 2015 and what still needs to be addressed?
Matthew G Hanna, Liron Pantanowitz, Andrew J Evans.
J Clin Pathol, 2015 May 17; 68(7). PMID: 25979986
Review.
Whole slide imaging diagnostic concordance with light microscopy for breast needle biopsies.
W Scott Campbell, Steven H Hinrichs, +5 authors, William W West.
Hum Pathol, 2014 Jun 11; 45(8). PMID: 24913758
Pulmonary large cell carcinoma lacking squamous differentiation is clinicopathologically indistinguishable from solid-subtype adenocarcinoma.
David H Hwang, David P Szeto, +2 authors, Lynette M Sholl.
Arch Pathol Lab Med, 2013 Jun 07; 138(5). PMID: 23738762
Digital reporting of whole-slide images is safe and suitable for assessing organ quality in preimplantation renal biopsies.
Albino Eccher, Desley Neil, +9 authors, Matteo Brunelli.
Hum Pathol, 2015 Nov 09; 47(1). PMID: 26547252
Validating whole slide imaging for diagnostic purposes in pathology: guideline from the College of American Pathologists Pathology and Laboratory Quality Center.
Liron Pantanowitz, John H Sinard, +8 authors, College of American Pathologists Pathology and Laboratory Quality Center.
Arch Pathol Lab Med, 2013 May 03; 137(12). PMID: 23634907    Free PMC article.
Highly Cited. Review.
The evaluation of colon biopsies using virtual microscopy is reliable.
Rachel S van der Post, Jeroen A W M van der Laak, +4 authors, Marius Nap.
Histopathology, 2013 May 23; 63(1). PMID: 23692065
Validation of diagnostic accuracy with whole-slide imaging compared with glass slide review in dermatopathology.
Kabeer K Shah, Julia S Lehman, +3 authors, Carilyn N Wieland.
J Am Acad Dermatol, 2016 Oct 16; 75(6). PMID: 27742174
Whole slide images as a platform for initial diagnostics in histopathology in a medium-sized routine laboratory.
Shaimaa Al-Janabi, André Huisman, +2 authors, Paul J van Diest.
J Clin Pathol, 2012 Oct 25; 65(12). PMID: 23093511
Whole slide images for primary diagnostics of gastrointestinal tract pathology: a feasibility study.
Shaimaa Al-Janabi, André Huisman, +4 authors, Paul J van Diest.
Hum Pathol, 2011 Sep 23; 43(5). PMID: 21937077
Clinical examination and validation of primary diagnosis in anatomic pathology using whole slide digital images.
Drazen M Jukić, Laura M Drogowski, Jamie Martina, Anil V Parwani.
Arch Pathol Lab Med, 2011 Mar 04; 135(3). PMID: 21366463
Whole slide images for primary diagnostics of paediatric pathology specimens: a feasibility study.
Shaimaa Al-Janabi, André Huisman, +2 authors, Paul J van Diest.
J Clin Pathol, 2012 Dec 04; 66(3). PMID: 23204560
Virtual surgical pathology in underdeveloped countries: The Zambia Project.
Fabio Pagni, Francesca Bono, +2 authors, Anna Cappellini.
Arch Pathol Lab Med, 2011 Feb 03; 135(2). PMID: 21284441
Enabling digital pathology in the diagnostic setting: navigating through the implementation journey in an academic medical centre.
Chee Leong Cheng, Rafay Azhar, +7 authors, Puay Hoon Tan.
J Clin Pathol, 2016 Feb 14; 69(9). PMID: 26873939
Evaluation of 2 whole-slide imaging applications in dermatopathology.
Nicole Velez, Drazen Jukic, Jonhan Ho.
Hum Pathol, 2008 Jul 11; 39(9). PMID: 18614200
Creation of a fully digital pathology slide archive by high-volume tissue slide scanning.
André Huisman, Arnoud Looijen, Steven M van den Brink, Paul J van Diest.
Hum Pathol, 2010 Feb 05; 41(5). PMID: 20129646
Multi-institutional comparison of whole slide digital imaging and optical microscopy for interpretation of hematoxylin-eosin-stained breast tissue sections.
Savitri Krishnamurthy, Kurt Mathews, +12 authors, Kevin Liang.
Arch Pathol Lab Med, 2013 Aug 21; 137(12). PMID: 23947655
The Diagnostic Concordance of Whole Slide Imaging and Light Microscopy: A Systematic Review.
Edward Goacher, Rebecca Randell, Bethany Williams, Darren Treanor.
Arch Pathol Lab Med, 2016 Jul 12; 141(1). PMID: 27399211
Systematic Review.
Whole slide images for primary diagnostics of urinary system pathology: a feasibility study.
Shaimaa Al-Janabi, André Huisman, +3 authors, Paul J van Diest.
J Renal Inj Prev, 2015 Jan 23; 3(4). PMID: 25610886    Free PMC article.
Whole slide images for primary diagnostics in dermatopathology: a feasibility study.
Shaimaa Al-Janabi, André Huisman, +5 authors, Paul J van Diest.
J Clin Pathol, 2011 Oct 28; 65(2). PMID: 22031590
An array microscope for ultrarapid virtual slide processing and telepathology. Design, fabrication, and validation study.
Ronald S Weinstein, Michael R Descour, +15 authors, Peter H Bartels.
Hum Pathol, 2005 Jan 26; 35(11). PMID: 15668886
Commentary: Whole-slide Images - Good Enough for Primary Diagnosis?
Thomas W Bauer.
J Pathol Inform, 2018 Mar 14; 9. PMID: 29531848    Free PMC article.
Will Digital Pathology be as Disruptive as Genomics?
Steven N Hart.
J Pathol Inform, 2018 Sep 01; 9. PMID: 30167342    Free PMC article.
Conventional Microscopical versus Digital Whole-Slide Imaging-Based Diagnosis of Thin-Layer Cervical Specimens: A Validation Study.
Odille Bongaerts, Carla Clevers, +7 authors, Marius Nap.
J Pathol Inform, 2018 Sep 11; 9. PMID: 30197818    Free PMC article.
Validation of Remote Digital Frozen Sections for Cancer and Transplant Intraoperative Services.
Luca Cima, Matteo Brunelli, +14 authors, Albino Eccher.
J Pathol Inform, 2018 Nov 20; 9. PMID: 30450263    Free PMC article.
Implementing the DICOM Standard for Digital Pathology.
Markus D Herrmann, David A Clunie, +19 authors, Jochen K Lennerz.
J Pathol Inform, 2018 Dec 12; 9. PMID: 30533276    Free PMC article.
Artificial intelligence in digital pathology - new tools for diagnosis and precision oncology.
Kaustav Bera, Kurt A Schalper, +2 authors, Anant Madabhushi.
Nat Rev Clin Oncol, 2019 Aug 11; 16(11). PMID: 31399699    Free PMC article.
Highly Cited. Review.
Head and Neck Cancer Detection in Digitized Whole-Slide Histology Using Convolutional Neural Networks.
Martin Halicek, Maysam Shahedi, +4 authors, Baowei Fei.
Sci Rep, 2019 Oct 03; 9(1). PMID: 31575946    Free PMC article.
Translational AI and Deep Learning in Diagnostic Pathology.
Ahmed Serag, Adrian Ion-Margineanu, +5 authors, Peter Hamilton.
Front Med (Lausanne), 2019 Oct 22; 6. PMID: 31632973    Free PMC article.
Review.
Next generation diagnostic pathology: use of digital pathology and artificial intelligence tools to augment a pathological diagnosis.
Anil V Parwani.
Diagn Pathol, 2019 Dec 29; 14(1). PMID: 31881972    Free PMC article.
Whole-Slide Image Focus Quality: Automatic Assessment and Impact on AI Cancer Detection.
Timo Kohlberger, Yun Liu, +5 authors, Martin C Stumpe.
J Pathol Inform, 2020 Jan 11; 10. PMID: 31921487    Free PMC article.
A Method to Reuse Archived H&E Stained Histology Slides for a Multiplex Protein Biomarker Analysis.
James P Hinton, Katerina Dvorak, +5 authors, Raymond B Nagle.
Methods Protoc, 2019 Nov 17; 2(4). PMID: 31731599    Free PMC article.
Whole Slide Imaging and Its Applications to Histopathological Studies of Liver Disorders.
Rossana C N Melo, Maximilian W D Raas, +3 authors, Thiago P Silva.
Front Med (Lausanne), 2020 Jan 24; 6. PMID: 31970160    Free PMC article.
Review.
Deep Learning Models for Histopathological Classification of Gastric and Colonic Epithelial Tumours.
Osamu Iizuka, Fahdi Kanavati, +3 authors, Masayuki Tsuneki.
Sci Rep, 2020 Feb 01; 10(1). PMID: 32001752    Free PMC article.
Whole slide imaging compared with light microscopy for primary diagnosis in surgical neuropathology: a validation study.
Ali Alassiri, Amna Almutrafi, +5 authors, Walid Khalbuss.
Ann Saudi Med, 2020 Feb 07; 40(1). PMID: 32026707    Free PMC article.
Impact of Deep Learning Assistance on the Histopathologic Review of Lymph Nodes for Metastatic Breast Cancer.
David F Steiner, Robert MacDonald, +6 authors, Martin C Stumpe.
Am J Surg Pathol, 2018 Oct 13; 42(12). PMID: 30312179    Free PMC article.
Highly Cited.
Precise Identification of Cell and Tissue Features Important for Histopathologic Diagnosis by a Whole Slide Imaging System.
Thomas W Bauer, Cynthia Behling, +6 authors, Jinsong Qiu.
J Pathol Inform, 2020 Mar 11; 11. PMID: 32154040    Free PMC article.
Introduction to digital pathology and computer-aided pathology.
Soojeong Nam, Yosep Chong, +5 authors, Heounjeong Go.
J Pathol Transl Med, 2020 Feb 13; 54(2). PMID: 32045965    Free PMC article.
Review.
["Digital Pathology in Diagnostics-reporting on digital images" guideline of the Professional Association of German Pathologists].
G Haroske, R Zwönitzer, P Hufnagl, Kommission Digitale Pathologie.
Pathologe, 2018 Nov 07; 39(Suppl 2). PMID: 30397786
Review.
Enhancing the Value of Histopathological Assessment of Allograft Biopsy Monitoring.
Michelle A Wood-Trageser, Andrew J Lesniak, Anthony J Demetris.
Transplantation, 2019 Feb 16; 103(7). PMID: 30768568    Free PMC article.
Review.
Validation of a digital pathology system including remote review during the COVID-19 pandemic.
Matthew G Hanna, Victor E Reuter, +21 authors, Meera Hameed.
Mod Pathol, 2020 Jun 24; 33(11). PMID: 32572154    Free PMC article.
Artificial intelligence in digital breast pathology: Techniques and applications.
Asmaa Ibrahim, Paul Gamble, +4 authors, Emad A Rakha.
Breast, 2020 Jan 15; 49. PMID: 31935669    Free PMC article.
Review.
A deep learning model to predict RNA-Seq expression of tumours from whole slide images.
Benoît Schmauch, Alberto Romagnoni, +11 authors, Gilles Wainrib.
Nat Commun, 2020 Aug 05; 11(1). PMID: 32747659    Free PMC article.
Histologic Classification and Molecular Signature of Polymorphous Adenocarcinoma (PAC) and Cribriform Adenocarcinoma of Salivary Gland (CASG): An International Interobserver Study.
Bin Xu, Andrea L Barbieri, +26 authors, Nora Katabi.
Am J Surg Pathol, 2020 Jan 10; 44(4). PMID: 31917707    Free PMC article.
The Importance of eSlide Macro Images for Primary Diagnosis with Whole Slide Imaging.
Filippo Fraggetta, Yukako Yagi, +6 authors, Liron Pantanowitz.
J Pathol Inform, 2019 Jan 22; 9. PMID: 30662792    Free PMC article.
Clinically applicable histopathological diagnosis system for gastric cancer detection using deep learning.
Zhigang Song, Shuangmei Zou, +21 authors, Huaiyin Shi.
Nat Commun, 2020 Aug 29; 11(1). PMID: 32855423    Free PMC article.
Interactive thyroid whole slide image diagnostic system using deep representation.
Pingjun Chen, Xiaoshuang Shi, +3 authors, Paul D Gader.
Comput Methods Programs Biomed, 2020 Jul 08; 195. PMID: 32634647    Free PMC article.
Twenty Years of Digital Pathology: An Overview of the Road Travelled, What is on the Horizon, and the Emergence of Vendor-Neutral Archives.
Liron Pantanowitz, Ashish Sharma, +3 authors, Joel Saltz.
J Pathol Inform, 2019 Jan 05; 9. PMID: 30607307    Free PMC article.
Review.
Parathyroid Frozen Section Interpretation via Desktop Telepathology Systems: A Validation Study.
Edward Chandraratnam, Leonardo D Santos, +4 authors, Ronald Y Chin.
J Pathol Inform, 2019 Jan 05; 9. PMID: 30607308    Free PMC article.
Invention and Early History of Telepathology (1985-2000).
Ronald S Weinstein, Michael J Holcomb, Elizabeth A Krupinski.
J Pathol Inform, 2019 Feb 21; 10. PMID: 30783545    Free PMC article.
Review.
Similar image search for histopathology: SMILY.
Narayan Hegde, Jason D Hipp, +11 authors, Martin C Stumpe.
NPJ Digit Med, 2019 Jul 16; 2. PMID: 31304402    Free PMC article.
Agreement in Histological Assessment of Mitotic Activity Between Microscopy and Digital Whole Slide Images Informs Conversion for Clinical Diagnosis.
Bih-Rong Wei, Charles H Halsey, +21 authors, R Mark Simpson.
Acad Pathol, 2019 Jul 20; 6. PMID: 31321298    Free PMC article.
Artificial intelligence (AI) and big data in cancer and precision oncology.
Zodwa Dlamini, Flavia Zita Francies, Rodney Hull, Rahaba Marima.
Comput Struct Biotechnol J, 2020 Oct 01; 18. PMID: 32994889    Free PMC article.
Review.
Artificial intelligence in gastric cancer: Application and future perspectives.
Peng-Hui Niu, Lu-Lu Zhao, +2 authors, Ying-Tai Chen.
World J Gastroenterol, 2020 Oct 08; 26(36). PMID: 33024393    Free PMC article.
Review.
A Regulatory Science Initiative to Harmonize and Standardize Digital Pathology and Machine Learning Processes to Speed up Clinical Innovation to Patients.
Hetal Desai Marble, Richard Huang, +14 authors, Jochen K Lennerz.
J Pathol Inform, 2020 Oct 13; 11. PMID: 33042601    Free PMC article.
Whole Slide Imaging (WSI) in Pathology: Current Perspectives and Future Directions.
Neeta Kumar, Ruchika Gupta, Sanjay Gupta.
J Digit Imaging, 2020 May 30; 33(4). PMID: 32468487    Free PMC article.
Review.
Recommendations for pathologic practice using digital pathology: consensus report of the Korean Society of Pathologists.
Yosep Chong, Dae Cheol Kim, +5 authors, Medical Informatics Study Group of the Korean Society of Pathologists.
J Pathol Transl Med, 2020 Oct 08; 54(6). PMID: 33027850    Free PMC article.
Review.
A narrative review of digital pathology and artificial intelligence: focusing on lung cancer.
Taro Sakamoto, Tomoi Furukawa, +8 authors, Junya Fukuoka.
Transl Lung Cancer Res, 2020 Nov 20; 9(5). PMID: 33209648    Free PMC article.
Review.
Digital Pathology: Advantages, Limitations and Emerging Perspectives.
Stephan W Jahn, Markus Plass, Farid Moinfar.
J Clin Med, 2020 Nov 22; 9(11). PMID: 33217963    Free PMC article.
Review.
The performance of digital microscopy for primary diagnosis in human pathology: a systematic review.
Anna Luíza Damaceno Araújo, Lady Paola Aristizábal Arboleda, +13 authors, Alan Roger Santos-Silva.
Virchows Arch, 2019 Jan 28; 474(3). PMID: 30685784
Systematic Review.
Deep neural network models for computational histopathology: A survey.
Chetan L Srinidhi, Ozan Ciga, Anne L Martel.
Med Image Anal, 2020 Oct 14; 67. PMID: 33049577    Free PMC article.
A large-scale internal validation study of unsupervised virtual trichrome staining technologies on nonalcoholic steatohepatitis liver biopsies.
Joshua J Levy, Nasim Azizgolshani, +7 authors, Louis J Vaickus.
Mod Pathol, 2020 Dec 11; 34(4). PMID: 33299110    Free PMC article.
Interobserver variation in the classification of thymic lesions including biopsies and resection specimens in an international digital microscopy panel.
Janina L Wolf, Francien van Nederveen, +8 authors, Michael A den Bakker.
Histopathology, 2020 Jun 09; 77(5). PMID: 32506527    Free PMC article.
(Re) Defining the High-Power Field for Digital Pathology.
David Kim, Liron Pantanowitz, +6 authors, Matthew G Hanna.
J Pathol Inform, 2020 Dec 22; 11. PMID: 33343994    Free PMC article.
Using Image Registration and Machine Learning to Develop a Workstation Tool for Rapid Analysis of Glomeruli in Medical Renal Biopsies.
David C Wilbur, Jason R Pettus, +4 authors, Eric Wirch.
J Pathol Inform, 2020 Dec 22; 11. PMID: 33343997    Free PMC article.
SuperHistopath: A Deep Learning Pipeline for Mapping Tumor Heterogeneity on Low-Resolution Whole-Slide Digital Histopathology Images.
Konstantinos Zormpas-Petridis, Rosa Noguera, +3 authors, Yinyin Yuan.
Front Oncol, 2021 Feb 09; 10. PMID: 33552964    Free PMC article.
Harnessing non-destructive 3D pathology.
Jonathan T C Liu, Adam K Glaser, +4 authors, Anant Madabhushi.
Nat Biomed Eng, 2021 Feb 17; 5(3). PMID: 33589781    Free PMC article.
Review.
Deep learning powers cancer diagnosis in digital pathology.
Yunjie He, Hong Zhao, Stephen T C Wong.
Comput Med Imaging Graph, 2021 Jan 17; 88. PMID: 33453648    Free PMC article.
Review.
Interobserver variability in upfront dichotomous histopathological assessment of ductal carcinoma in situ of the breast: the DCISion study.
Hélène Dano, Serdar Altinay, +37 authors, Mieke R Van Bockstal.
Mod Pathol, 2019 Sep 20; 33(3). PMID: 31534203    Free PMC article.
Digital Pathology During the COVID-19 Outbreak in Italy: Survey Study.
Simone Giaretto, Salvatore Lorenzo Renne, +19 authors, Luca Di Tommaso.
J Med Internet Res, 2021 Jan 28; 23(2). PMID: 33503002    Free PMC article.
Rapid Validation of Whole-Slide Imaging for Primary Histopathology Diagnosis.
Megan I Samuelson, Stephanie J Chen, +5 authors, Anand Rajan K D.
Am J Clin Pathol, 2021 Jan 30; 155(5). PMID: 33511392    Free PMC article.
Discrepancies in digital hematopathology diagnoses for consultation and expert panel analysis.
Michiel van den Brand, Peet T G A Nooijen, +6 authors, Konnie M Hebeda.
Virchows Arch, 2020 Aug 26; 478(3). PMID: 32840673    Free PMC article.
Implementation of Collodion Bag Protocol to Improve Whole-slide Imaging of Scant Gynecologic Curettage Specimens.
Iny Jhun, David Levy, +9 authors, Eric Joon Yang.
J Pathol Inform, 2021 May 21; 12. PMID: 34012706    Free PMC article.
Remote Reporting from Home for Primary Diagnosis in Surgical Pathology: A Tertiary Oncology Center Experience during the COVID-19 Pandemic.
Vidya Rao, Rajiv Kumar, +17 authors, Sangeeta Desai.
J Pathol Inform, 2021 May 21; 12. PMID: 34012707    Free PMC article.
Verification and Validation of Digital Pathology (Whole Slide Imaging) for Primary Histopathological Diagnosis: All Wales Experience.
M Babawale, A Gunavardhan, +24 authors, Muhammad Aslam.
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Selection of Representative Histologic Slides in Interobserver Reproducibility Studies: Insights from Expert Review for Ovarian Carcinoma Subtype Classification.
Marios A Gavrielides, Brigitte M Ronnett, +2 authors, Jeffrey D Seidman.
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Conventional and semi-automatic histopathological analysis of tumor cell content for multigene sequencing of lung adenocarcinoma.
Daniel Kazdal, Eugen Rempel, +29 authors, Mark Kriegsmann.
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Clinical applications of artificial intelligence and machine learning in cancer diagnosis: looking into the future.
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Review.
Digital Versus Optical Diagnosis of Follicular Patterned Thyroid Lesions.
Ayat Aloqaily, Antonio Polonia, +4 authors, Catarina Eloy.
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