. 2019 Jan;16(5).
doi: 10.1038/s41571-019-0166-8.

CRISPR-Cas: a tool for cancer research and therapeutics

Hao Yin 1 Wen Xue 2 Daniel G Anderson 3 
  • PMID: 30664678
  •     23 citations


In the past decade, the development of a genome-editing technology mediated by CRISPR has made genetic engineering easier than ever, both in vitro and in vivo. CRISPR systems have enabled important advances in cancer research by accelerating the development of study models or as a tool in genetic screening studies, including those aiming to discover and validate therapeutic targets. In this Review, we discuss these applications as well as new potential uses of CRISPR to assist in cancer detection or the development of anticancer therapies.

CRISPR/Cas9 Editing of the Polyomavirus Tumor Antigens Inhibits Merkel Cell Carcinoma Growth In Vitro.
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Cancers (Basel), 2019 Aug 31; 11(9). PMID: 31466237    Free PMC article.
Polymer-stabilized Cas9 nanoparticles and modified repair templates increase genome editing efficiency.
David N Nguyen, Theodore L Roth, +11 authors, Alexander Marson.
Nat Biotechnol, 2019 Dec 11; 38(1). PMID: 31819258    Free PMC article.
TAG-RNAi overcomes off-target effects in cancer models.
Julien Champagne, Laetitia K Linares, +7 authors, Frédéric Bienvenu.
Oncogene, 2019 Sep 29; 39(4). PMID: 31558799
Specificity Assessment of CRISPR Genome Editing of Oncogenic EGFR Point Mutation with Single-Base Differences.
Taegeun Bae, Hanseop Kim, +4 authors, Junho K Hur.
Molecules, 2019 Dec 28; 25(1). PMID: 31877894    Free PMC article.
Systems biology: perspectives on multiscale modeling in research on endocrine-related cancers.
Robert Clarke, John J Tyson, +4 authors, Yue Wang.
Endocr Relat Cancer, 2019 Apr 10; 26(6). PMID: 30965282    Free PMC article.
CRISPR-SONIC: targeted somatic oncogene knock-in enables rapid in vivo cancer modeling.
Haiwei Mou, Deniz M Ozata, +7 authors, Wen Xue.
Genome Med, 2019 Apr 17; 11(1). PMID: 30987660    Free PMC article.
Rapid detection of African swine fever virus using Cas12a-based portable paper diagnostics.
Shuhan Lu, Fang Li, +7 authors, Hao Yin.
Cell Discov, 2020 Apr 15; 6. PMID: 32284877    Free PMC article.
Advances in CRISPR/Cas-based Gene Therapy in Human Genetic Diseases.
Shao-Shuai Wu, Qing-Cui Li, +2 authors, Chun-Qing Song.
Theranostics, 2020 Apr 16; 10(10). PMID: 32292501    Free PMC article.
Gene Therapy in Cancer Treatment: Why Go Nano?
Catarina Roma-Rodrigues, Lorenzo Rivas-García, Pedro V Baptista, Alexandra R Fernandes.
Pharmaceutics, 2020 Mar 11; 12(3). PMID: 32151052    Free PMC article.
p53 modifications: exquisite decorations of the powerful guardian.
Yanqing Liu, Omid Tavana, Wei Gu.
J Mol Cell Biol, 2019 Jul 10; 11(7). PMID: 31282934    Free PMC article.
Prevention of Avian Retrovirus Infection in Chickens Using CRISPR-Cas9 Delivered by Marek's Disease Virus.
Kai Li, Yongzhen Liu, +10 authors, Xiaomei Wang.
Mol Ther Nucleic Acids, 2020 Jul 11; 21. PMID: 32650233    Free PMC article.
CRISPR and transposon in vivo screens for cancer drivers and therapeutic targets.
Imran Noorani, Allan Bradley, Jorge de la Rosa.
Genome Biol, 2020 Aug 20; 21(1). PMID: 32811551    Free PMC article.
CRISPR-cas9: a powerful tool towards precision medicine in cancer treatment.
Hui Xing, Ling-Hua Meng.
Acta Pharmacol Sin, 2019 Dec 04; 41(5). PMID: 31792341    Free PMC article.
CRISPR-Cas systems: Overview, innovations and applications in human disease research and gene therapy.
Yuanyuan Xu, Zhanjun Li.
Comput Struct Biotechnol J, 2020 Oct 03; 18. PMID: 33005303    Free PMC article.
Combined application of CRISPR-Cas and stem cells for clinical and basic research.
Meng Yan, Jinsong Li.
Cell Regen, 2020 Oct 10; 9(1). PMID: 33033974    Free PMC article.
CRISPR/Cas: From Tumor Gene Editing to T Cell-Based Immunotherapy of Cancer.
Mohammadreza Azangou-Khyavy, Mobina Ghasemi, +4 authors, Jafar Kiani.
Front Immunol, 2020 Oct 30; 11. PMID: 33117331    Free PMC article.
In silico Method in CRISPR/Cas System: An Expedite and Powerful Booster.
Yuwei Zhang, Guofang Zhao, +4 authors, Qi Liao.
Front Oncol, 2020 Oct 31; 10. PMID: 33123486    Free PMC article.
CRISPR-engineered human brown-like adipocytes prevent diet-induced obesity and ameliorate metabolic syndrome in mice.
Chih-Hao Wang, Morten Lundh, +16 authors, Yu-Hua Tseng.
Sci Transl Med, 2020 Aug 28; 12(558). PMID: 32848096    Free PMC article.
Cancer biology functional genomics: From small RNAs to big dreams.
Soumya Sundara Rajan, Katelyn R Ludwig, +2 authors, Natasha J Caplen.
Mol Carcinog, 2020 Oct 13; 59(12). PMID: 33043516    Free PMC article.
Designer nucleases to treat malignant cancers driven by viral oncogenes.
Tristan A Scott, Kevin V Morris.
Virol J, 2021 Jan 15; 18(1). PMID: 33441159    Free PMC article.
Leveraging Population Genomics for Individualized Correction of the Hallmarks of Alpha-1 Antitrypsin Deficiency.
Chao Wang, Pei Zhao, +2 authors, William E Balch.
Chronic Obstr Pulm Dis, 2020 Jul 30; 7(3). PMID: 32726074    Free PMC article.
Recent advances in preclinical models for lung squamous cell carcinoma.
Yuanwang Pan, Han Han, +2 authors, Kwok-Kin Wong.
Oncogene, 2021 Mar 13; 40(16). PMID: 33707749
Delivery of Tissue-Targeted Scalpels: Opportunities and Challenges for In Vivo CRISPR/Cas-Based Genome Editing.
Tuo Wei, Qiang Cheng, +3 authors, Daniel J Siegwart.
ACS Nano, 2020 Jul 23; 14(8). PMID: 32697075    Free PMC article.