Review
. 2019 Aug; 10:21.
doi: 10.4103/jpi.jpi_27_19.

The Landscape of Digital Pathology in Transplantation: From the Beginning to the Virtual E-Slide

Ilaria Girolami 1 Anil Parwani 2 Valeria Barresi 1 Stefano Marletta 1 Serena Ammendola 1 Lavinia Stefanizzi 1 Luca Novelli 3 Arrigo Capitanio 4 Matteo Brunelli 1 Liron Pantanowitz 5 Albino Eccher 1 
Affiliations
  • PMID: 31367473
  •     104 References
  •     4 citations

Abstract

Background: Digital pathology has progressed over the last two decades, with many clinical and nonclinical applications. Transplantation pathology is a highly specialized field in which the majority of practicing pathologists do not have sufficient expertise to handle critical needs. In this context, digital pathology has proven to be useful as it allows for timely access to expert second-opinion teleconsultation. The aim of this study was to review the experience of the application of digital pathology to the field of transplantation.

Methods: Papers on this topic were retrieved using PubMed as a search engine. Inclusion criteria were the presence of transplantation setting and the use of any type of digital image with or without the use of image analysis tools; the search was restricted to English language papers published in the 25 years until December 31, 2018.

Results: Literature regarding digital transplant pathology is mostly about the digital interpretation of posttransplant biopsies (75 vs. 19), with 15/75 (20%) articles focusing on agreement/reproducibility. Several papers concentrated on the correlation between biopsy features assessed by digital image analysis (DIA) and clinical outcome (45/75, 60%). Whole-slide imaging (WSI) only appeared in recent publications, starting from 2011 (13/75, 17.3%). Papers dealing with preimplantation biopsy are less numerous, the majority (13/19, 68.4%) of which focus on diagnostic agreement between digital microscopy and light microscopy (LM), with WSI technology being used in only a small quota of papers (4/19, 21.1%).

Conclusions: Overall, published studies show good concordance between digital microscopy and LM modalities for diagnosis. DIA has the potential to increase diagnostic reproducibility and facilitate the identification and quantification of histological parameters. Thus, with advancing technology such as faster scanning times, better image resolution, and novel image algorithms, it is likely that WSI will eventually replace LM.

Keywords: Digital pathology; donor biopsy; graft biopsy; image analysis; transplantation.

Clinical rejection is distinguished from subclinical rejection by increased infiltration by a population of activated macrophages.
P C Grimm, R McKenna, +6 authors, D N Rush.
J Am Soc Nephrol, 1999 Jul 15; 10(7). PMID: 10405215
Computerized histomorphometric assessment of protocol renal transplant biopsy specimens for surrogate markers of chronic rejection.
M L Nicholson, E Bailey, +2 authors, P N Furness.
Transplantation, 1999 Aug 10; 68(2). PMID: 10440394
Acute rejection-associated tubular basement membrane defects and chronic allograft nephropathy.
S M Bonsib, S R Abul-Ezz, +4 authors, P D Walker.
Kidney Int, 2000 Oct 24; 58(5). PMID: 11044243
Prediction of chronic allograft failure using computerized image analysis of postperfusion biopsy specimen: study of cadaver kidney transplants.
T Furukawa, T Kinukawa, +2 authors, S Ohshima.
Transplant Proc, 2001 Mar 27; 33(1-2). PMID: 11267146
Transforming growth factor-beta1 expression in early biopsy specimen predicts long-term graft function following pediatric renal transplantation.
T Ishimura, M Fujisawa, +7 authors, S Kamidono.
Clin Transplant, 2001 Jun 08; 15(3). PMID: 11389709
Effect of ischemia-reperfusion on bile canalicular F-actin microfilaments in hepatocytes of human liver allograft: image analysis by confocal laser scanning microscopy.
L Benkoël, F Dodero, +5 authors, A Chamlian.
Dig Dis Sci, 2001 Aug 18; 46(8). PMID: 11508665
Development and experience with an integrated system for transplantation telepathology.
M I Minervini, Y Yagi, +6 authors, A Demetris.
Hum Pathol, 2002 Jan 05; 32(12). PMID: 11774166
Telepathology for the biopsy specimens from human allografted kidney: effectiveness and pitfalls.
H Ito, K Shomori, H Adachi, K Taniyama.
Clin Transplant, 2002 Jan 17; 15 Suppl 5. PMID: 11791797
Internet teleconferencing method for telepathology consultations from lung and heart transplant patients.
Alberto M Marchevsky, Sean K Lau, +4 authors, Michael C Fishbein.
Hum Pathol, 2002 Jun 11; 33(4). PMID: 12055675
Clinical relevance of immunohistochemical staining for ecto-AMPase and ecto-ATPase in chronic allograft nephropathy (CAN).
Kwok W Mui, Willem J van Son, +2 authors, Winston W Bakker.
Nephrol Dial Transplant, 2002 Dec 14; 18(1). PMID: 12480975
Glomerular size and global glomerulosclerosis in normal Caucasian donor kidneys: effects of aging and gender.
Mingxi Li, Kathy M Nicholls, Gavin J Becker.
J Nephrol, 2002 Dec 24; 15(6). PMID: 12495273
Computerized image analysis of Sirius Red-stained renal allograft biopsies as a surrogate marker to predict long-term allograft function.
Paul C Grimm, Peter Nickerson, +4 authors, David N Rush.
J Am Soc Nephrol, 2003 May 23; 14(6). PMID: 12761269
Focal segmental sclerotic lesions of the glomerulus in transplanted kidneys assessed using computerized image analysis.
Satoshi Sugiyama, Shinsuke Asano, +9 authors, Osamu Kamihira.
Clin Transplant, 2003 Jun 26; 17 Suppl 10. PMID: 12823254
Computer-assisted quantification of fibrosis in chronic allograft nephropaty by picosirius red-staining: a new tool for predicting long-term graft function.
Lars Pape, Thomas Henne, +4 authors, Paul C Grimm.
Transplantation, 2003 Sep 26; 76(6). PMID: 14508360
A morphometric insight into glomerular and interstitial lesions in acutely rejected renal allografts.
Marian Danilewicz, Małgorzata Wagrowska-Danilewicz.
Pol J Pathol, 2004 Jan 02; 54(3). PMID: 14703283
Expression of intercellular adhesion molecule-1 (ICAM- 1) during ischemia-reperfusion in human liver tissue allograft: image analysis by confocal laser scanning microscopy.
Liliane Benkoel, Frank Dodero, +6 authors, Dominique Lombardo.
Dig Dis Sci, 2004 Jan 07; 48(11). PMID: 14705823
Correlation of quantitative digital image analysis with the glomerular filtration rate in chronic allograft nephropathy.
Montserrat M Diaz Encarnacion, Matthew D Griffin, +4 authors, Joseph P Grande.
Am J Transplant, 2004 Feb 21; 4(2). PMID: 14974947
Immunohistochemical analysis of the interstitial mast cells in acute rejection of human renal allografts.
Marian Danilewicz, Małgorzata Wagrowska-Danilewicz.
Med Sci Monit, 2004 Apr 29; 10(5). PMID: 15114264
Assessment of donor liver steatosis: pathologist or automated software?
Hendrik Marsman, Takakazu Matsushita, +4 authors, Scott L Nyberg.
Hum Pathol, 2004 Apr 30; 35(4). PMID: 15116323
Computerized image analysis vs semiquantitative scoring in evaluation of kidney allograft fibrosis and prognosis.
Ståle Sund, Paul Grimm, Anna Varberg Reisaeter, Torstein Hovig.
Nephrol Dial Transplant, 2004 Sep 24; 19(11). PMID: 15385637
Effect of ischemia-reperfusion on Na+, K+-ATPase expression in human liver tissue allograft: image analysis by confocal laser scanning microscopy.
Liliane Benkoel, Frank Dodero, +6 authors, Dominique Lombardo.
Dig Dis Sci, 2004 Oct 16; 49(9). PMID: 15481308
Comparison of renal allograft fibrosis after transplantation from heart-beating and non-heart-beating donors.
J C Bains, R M Sandford, +3 authors, M L Nicholson.
Br J Surg, 2004 Dec 14; 92(1). PMID: 15593295
Renal arterial resistance index and computerized quantification of fibrosis as a combined predictive tool in chronic allograft nephropathy.
Lars Pape, Michael Mengel, +3 authors, Juergen Strehlau.
Pediatr Transplant, 2004 Dec 16; 8(6). PMID: 15598325
Methenamine silver staining quantitative digital histochemistry in chronic allograft nephropathy.
S Sarioglu, A Celik, +2 authors, D Tekis.
Transplant Proc, 2005 Feb 03; 36(10). PMID: 15686678
A descriptive study of small airway reticular basement membrane thickening in clinically stable lung transplant recipients.
Chris Ward, Anthony De Soyza, +3 authors, Paul Corris.
J Heart Lung Transplant, 2005 May 18; 24(5). PMID: 15896749
The morphological compensatory change of peritubular capillary network in chronic allograft rejection.
Shinichi Nishi, Naofumi Imai, +8 authors, Fumitake Gejyo.
Clin Transplant, 2005 Jun 16; 19 Suppl 14. PMID: 15955163
Mast cells and c-Kit expression in liver allograft rejection.
A M Ahmed El-Refaie, A D Burt.
Histopathology, 2005 Sep 24; 47(4). PMID: 16178892
Early changes in basement membrane thickness in airway walls post-lung transplantation.
Lucas Law, Ling Zheng, +5 authors, Trevor J Williams.
J Heart Lung Transplant, 2005 Oct 08; 24(10). PMID: 16210132
No difference in degree of interstitial Sirius red-stained area in serial biopsies from area under concentration-over-time curves-guided cyclosporine versus tacrolimus-treated renal transplant recipients at one year.
Ajda T Rowshani, Eduard M Scholten, +11 authors, Sandrine Florquin.
J Am Soc Nephrol, 2005 Nov 25; 17(1). PMID: 16306168
Correlative insights into the immunoexpression of transforming growth factor beta-1 in acutely rejected renal allografts.
Marian Danilewicz, Małgorzata Wagrowska-Danilewicz.
Pathol Res Pract, 2005 Dec 14; 202(1). PMID: 16343799
Expression of the chemokine receptor CXCR3 in human renal allografts--a prospective study.
Ute Hoffmann, Stephan Segerer, +5 authors, Bernhard K Krämer.
Nephrol Dial Transplant, 2006 Jan 20; 21(5). PMID: 16421159
Renal medullary changes in renal allograft recipients with raised serum creatinine.
B Sis, S Sarioglu, +5 authors, T Camsari.
J Clin Pathol, 2006 Feb 08; 59(4). PMID: 16461569    Free PMC article.
Molecular comparison of calcineurin inhibitor-induced fibrogenic responses in protocol renal transplant biopsies.
Marian C Roos-van Groningen, Eduard M Scholten, +9 authors, Michael Eikmans.
J Am Soc Nephrol, 2006 Feb 10; 17(3). PMID: 16467444
Nuclear mean gray level and chromatin distribution changes in cardiomyocytes of heart transplant recipients suffering from acute cellular rejection.
M Zakliczynski, J Nozynski, +3 authors, M Zembala.
Transplant Proc, 2006 Mar 01; 38(1). PMID: 16504738
Quantitative digital histochemistry with methenamine silver staining in renal allograft biopsies excluding pure chronic allograft nephropathy cases.
S Sarioglu, B Sis, +4 authors, T Camsari.
Transplant Proc, 2006 Mar 22; 38(2). PMID: 16549156
Endomyocardial infiltration by B and NK cells foreshadows the recurrence of cardiac allograft rejection.
C Sorrentino, A Scarinci, +5 authors, E Di Carlo.
J Pathol, 2006 Apr 04; 209(3). PMID: 16583358
An image analysis-based method for quantification of chronic allograft damage index parameters.
Jouni Lauronen, Pekka Häyry, Timo Paavonen.
APMIS, 2006 Jul 22; 114(6). PMID: 16856966
Untreated rejection in 6-month protocol biopsies is not associated with fibrosis in serial biopsies or with loss of graft function.
Eduard M Scholten, Ajda T Rowshani, +9 authors, Johan W de Fijter.
J Am Soc Nephrol, 2006 Aug 11; 17(9). PMID: 16899517
Quantification of interstitial fibrosis by image analysis on routine renal biopsy 1 year after transplantation in patients managed by C2 monitoring of cyclosporine microemulsion.
A Servais, V Meas-Yedid, +3 authors, E Thervet.
Transplant Proc, 2007 Oct 24; 39(8). PMID: 17954173
Thymoglobulin administered early after heart transplantation reduces early myocardial hypertrophy assessed by morphometric studies.
J Nozynski, M Zakliczynski, +5 authors, M Zembala.
Transplant Proc, 2007 Nov 21; 39(9). PMID: 18021995
Computer-assisted digital image analysis to quantify the mass and purity of isolated human islets before transplantation.
Nadja Niclauss, Antonino Sgroi, +7 authors, Domenico Bosco.
Transplantation, 2008 Dec 17; 86(11). PMID: 19077896
Computer-assisted image analysis of liver collagen: relationship to Ishak scoring and hepatic venous pressure gradient.
Vincenza Calvaruso, Andrew Kenneth Burroughs, +11 authors, Amar Paul Dhillon.
Hepatology, 2009 Jan 13; 49(4). PMID: 19133646
Validation of methodologies for quantifying isolated human islets: an Islet Cell Resources study.
H J Kissler, J C Niland, +10 authors, D B Kaufman.
Clin Transplant, 2009 Sep 02; 24(2). PMID: 19719726    Free PMC article.
Expression and role of integrin-linked kinase and collagen IV in human renal allografts with interstitial fibrosis and tubular atrophy.
Qiang Yan, Weiguo Sui, +5 authors, Hequn Zou.
Transpl Immunol, 2010 Apr 20; 23(1-2). PMID: 20398767
Review.
Nucleometric study of anisonucleosis, diabetes and oxidative damage in liver biopsies of orthotopic liver transplant recipients with chronic hepatitis C virus infection.
Grace Guzman, Rohini Chennuri, +4 authors, Tibor Valyi-Nagy.
Pathol Oncol Res, 2010 Sep 21; 17(2). PMID: 20853078
Enhanced resolution of interstitial fibrosis in pediatric renal allograft biopsies using image analysis of trichrome stain.
Patricia E Birk, John S Gill, Tom D Blydt-Hansen, Ian W Gibson.
Pediatr Transplant, 2010 Oct 16; 14(7). PMID: 20946518
Digital image analysis of liver collagen predicts clinical outcome of recurrent hepatitis C virus 1 year after liver transplantation.
Pinelopi Manousou, Amar P Dhillon, +11 authors, Andrew K Burroughs.
Liver Transpl, 2011 Feb 01; 17(2). PMID: 21280191
Factors increasing quantitative interstitial fibrosis from 0 hr to 1 year in living kidney transplant patients receiving tacrolimus.
Yoshiko Miura, Shigeru Satoh, +11 authors, Tomonori Habuchi.
Transplantation, 2011 Apr 01; 91(1). PMID: 21452412
Digital pathology evaluation of complement C4d component deposition in the kidney allograft biopsies is a useful tool to improve reproducibility of the scoring.
Ernesta Brazdziute, Arvydas Laurinavicius.
Diagn Pathol, 2011 Apr 22; 6 Suppl 1. PMID: 21489200    Free PMC article.
Interstitial fibrosis evolution on early sequential screening renal allograft biopsies using quantitative image analysis.
A Servais, V Meas-Yedid, +8 authors, E Thervet.
Am J Transplant, 2011 Jun 16; 11(7). PMID: 21672152
A web-based pilot study of inter-pathologist reproducibility using the ISHLT 2004 working formulation for biopsy diagnosis of cardiac allograft rejection: the European experience.
Annalisa Angelini, Claus Boegelund Andersen, +21 authors, Margaret Burke.
J Heart Lung Transplant, 2011 Aug 06; 30(11). PMID: 21816625
Fractal dimension in quantifying the degree of myocardial cellular rejection after cardiac transplantation.
Roberto Douglas Moreira, Antonio Roberto Moriel, +2 authors, Moacir Fernandes de Godoy.
Rev Bras Cir Cardiovasc, 2011 Sep 07; 26(2). PMID: 21894404
New computerized color image analysis for the quantification of interstitial fibrosis in renal transplantation.
Vannary Meas-Yedid, Aude Servais, +9 authors, Emmanuel Morelon.
Transplantation, 2011 Sep 20; 92(8). PMID: 21926945
Superiority of virtual microscopy versus light microscopy in transplantation pathology.
Yasemin Ozluk, Paula L Blanco, +3 authors, Banu Sis.
Clin Transplant, 2011 Oct 01; 26(2). PMID: 21955102
Digital transplantation pathology: combining whole slide imaging, multiplex staining and automated image analysis.
K Isse, A Lesniak, +3 authors, A J Demetris.
Am J Transplant, 2011 Nov 08; 12(1). PMID: 22053785    Free PMC article.
Review.
Expression of GSK-3β in renal allograft tissue and its significance in pathogenesis of chronic allograft dysfunction.
Qiang Yan, Baoyao Wang, +4 authors, Hequn Zou.
Diagn Pathol, 2012 Jan 17; 7. PMID: 22243669    Free PMC article.
Longitudinal evaluation of microvessel density in survivors vs. nonsurvivors of cardiac pathologic antibody-mediated rejection.
Monica P Revelo, Dylan V Miller, +9 authors, Abdallah G Kfoury.
Cardiovasc Pathol, 2012 Mar 03; 21(6). PMID: 22381397
Liver collagen proportionate area predicts decompensation in patients with recurrent hepatitis C virus cirrhosis after liver transplantation.
Vincenza Calvaruso, Amar Paul Dhillon, +6 authors, Andrew Kenneth Burroughs.
J Gastroenterol Hepatol, 2012 Mar 22; 27(7). PMID: 22432427
Frozen section diagnosis in donor liver biopsies: observer variation of semiquantitative and quantitative steatosis assessment.
Stefan Biesterfeld, Jasmin Knapp, +3 authors, Gerd Otto.
Virchows Arch, 2012 Jul 10; 461(2). PMID: 22772768
Cellular infiltrates and NFκB subunit c-Rel signaling in kidney allografts of patients with clinical operational tolerance.
Luis E Becker, Fúvia de Oliveira Biazotto, +8 authors, Christian Morath.
Transplantation, 2012 Sep 08; 94(7). PMID: 22955189
Expression of MMP-2 and TIMP-1 in renal tissue of patients with chronic active antibody-mediated renal graft rejection.
Qiang Yan, Weiguo Sui, +3 authors, Hao Luo.
Diagn Pathol, 2012 Oct 13; 7. PMID: 23057632    Free PMC article.
Digital imaging in pathology.
Seung Park, Liron Pantanowitz, Anil Vasdev Parwani.
Clin Lab Med, 2012 Oct 20; 32(4). PMID: 23078660
Review.
Reliability of whole slide images as a diagnostic modality for renal allograft biopsies.
Kuang-Yu Jen, Jean L Olson, +3 authors, Zoltan G Laszik.
Hum Pathol, 2012 Dec 04; 44(5). PMID: 23199528
Digital image analysis of collagen assessment of progression of fibrosis in recurrent HCV after liver transplantation.
Pinelopi Manousou, Andrew K Burroughs, +13 authors, Amar Paul Dhillon.
J Hepatol, 2012 Dec 25; 58(5). PMID: 23262247
Experience with multimodality telepathology at the University of Pittsburgh Medical Center.
Liron Pantanowitz, Clayton A Wiley, +5 authors, Anil V Parwani.
J Pathol Inform, 2013 Feb 02; 3. PMID: 23372986    Free PMC article.
Impact of donor benign intimal thickening on cardiac allograft vasculopathy.
Jennifer J Devitt, Alexandra Rice, +3 authors, Timothy D G Lee.
J Heart Lung Transplant, 2013 Mar 19; 32(4). PMID: 23498165
Validating whole slide imaging for diagnostic purposes in pathology: guideline from the College of American Pathologists Pathology and Laboratory Quality Center.
Liron Pantanowitz, John H Sinard, +8 authors, College of American Pathologists Pathology and Laboratory Quality Center.
Arch Pathol Lab Med, 2013 May 03; 137(12). PMID: 23634907    Free PMC article.
Highly Cited. Review.
Automated digital image analysis of islet cell mass using Nikon's inverted eclipse Ti microscope and software to improve engraftment may help to advance the therapeutic efficacy and accessibility of islet transplantation across centers.
Valery Gmyr, Caroline Bonner, +10 authors, Julie Kerr-Conte.
Cell Transplant, 2013 May 21; 24(1). PMID: 23683575
Early changes in scores of chronic damage on transplant kidney protocol biopsies reflect donor characteristics, but not future graft function.
Ben Caplin, Kristin Veighey, +10 authors, Peter Veitch.
Clin Transplant, 2013 Oct 15; 27(6). PMID: 24118300    Free PMC article.
Automated image analysis method for detecting and quantifying macrovesicular steatosis in hematoxylin and eosin-stained histology images of human livers.
Nir I Nativ, Alvin I Chen, +8 authors, Martin L Yarmush.
Liver Transpl, 2013 Dec 18; 20(2). PMID: 24339411    Free PMC article.
Improved access to histopathology using a digital system could increase the organ donor pool and improve allocation.
Desley A H Neil, Ian S D Roberts, +2 authors, James M Neuberger.
Transpl Int, 2014 Mar 19; 27(8). PMID: 24629110
Review.
Banff fibrosis study: multicenter visual assessment and computerized analysis of interstitial fibrosis in kidney biopsies.
A B Farris, S Chan, +6 authors, M Mengel.
Am J Transplant, 2014 Apr 10; 14(4). PMID: 24712330
Application of Digital Image Analysis to Determine Pancreatic Islet Mass and Purity in Clinical Islet Isolation and Transplantation.
Ling-Jia Wang, Hermann J Kissler, +10 authors, Piotr Witkowski.
Cell Transplant, 2014 May 09; 24(7). PMID: 24806436    Free PMC article.
Digital pathology services in acute surgical situations.
D A H Neil, A J Demetris.
Br J Surg, 2014 Jun 12; 101(10). PMID: 24916616
Everolimus prevents coronary microvasculopathy in heart transplant recipients with normal coronary angiograms: an anatomo-functional study.
F Tona, M Fedrigo, +10 authors, A Angelini.
Transplant Proc, 2014 Sep 23; 46(7). PMID: 25242783
Fractal analysis of heart graft acute rejection microscopic images.
M Pijet, J Nozynski, +3 authors, M Zembala.
Transplant Proc, 2014 Nov 09; 46(8). PMID: 25380937
The Digital Microscopy in Organ Transplantation: Ergonomics of the Tele-Pathological Evaluation of Renal and Liver Grafts.
Constantinos S Mammas, Andreas Lazaris, +2 authors, Eustratios Patsouris.
Stud Health Technol Inform, 2015 Jul 15; 213. PMID: 26153017
Digital reporting of whole-slide images is safe and suitable for assessing organ quality in preimplantation renal biopsies.
Albino Eccher, Desley Neil, +9 authors, Matteo Brunelli.
Hum Pathol, 2015 Nov 09; 47(1). PMID: 26547252
Renal Graft Fibrosis and Inflammation Quantification by an Automated Fourier-Transform Infrared Imaging Technique.
Vincent Vuiblet, Michael Fere, +3 authors, Philippe Rieu.
J Am Soc Nephrol, 2015 Dec 20; 27(8). PMID: 26683669    Free PMC article.
Sirt1-Positive Lymphocytes in Acute Cellular Cardiac Allograft Rejection: Contributor to Pathogenesis and a Therapeutic Target.
Kerry J Welsh, Bihong Zhao, L Maximilian Buja, Robert E Brown.
ASAIO J, 2016 Jan 16; 62(3). PMID: 26771391
Granular swollen epithelial cells in the kidney allograft: A clinicopathological study with special emphasis on possible marker for kidney allograft aging.
Shigeo Hara, Takeshi Ishimura, +2 authors, Tomoo Itoh.
Nephrology (Carlton), 2016 Mar 13; 21 Suppl 1. PMID: 26969019
Increased hepatic progenitor cell response and ductular reaction in patients with severe recurrent HCV post-liver transplantation.
Seth N Sclair, Maria Isabel Fiel, +3 authors, Thomas D Schiano.
Clin Transplant, 2016 Mar 31; 30(6). PMID: 27027987
Fully Automated Islet Cell Counter (ICC) for the Assessment of Islet Mass, Purity, and Size Distribution by Digital Image Analysis.
Peter Buchwald, Andres Bernal, +3 authors, Camillo Ricordi.
Cell Transplant, 2016 May 20; 25(10). PMID: 27196960
Banff Histopathological Consensus Criteria for Preimplantation Kidney Biopsies.
H Liapis, J P Gaut, +18 authors, Banff Working Group.
Am J Transplant, 2016 Jun 23; 17(1). PMID: 27333454    Free PMC article.
The Diagnostic Concordance of Whole Slide Imaging and Light Microscopy: A Systematic Review.
Edward Goacher, Rebecca Randell, Bethany Williams, Darren Treanor.
Arch Pathol Lab Med, 2016 Jul 12; 141(1). PMID: 27399211
Systematic Review.
Virtual microscopy improves sharing of deceased donor kidneys.
Adena J Osband, Billie Fyfe, David A Laskow.
Am J Surg, 2016 Aug 18; 212(4). PMID: 27530976
Expression and Significance of RANTES and MCP-1 in Renal Tissue With Chronic Renal Allograft Dysfunction.
Q Yan, H Jiang, +3 authors, G Zou.
Transplant Proc, 2016 Aug 30; 48(6). PMID: 27569940
Mobile Health in Solid Organ Transplant: The Time Is Now.
J N Fleming, D J Taber, +2 authors, F Treiber.
Am J Transplant, 2017 Feb 12; 17(9). PMID: 28188681
Macrophage density in early surveillance biopsies predicts future renal transplant function.
Jan Hinrich Bräsen, Abedalrazag Khalifa, +8 authors, Sibylle von Vietinghoff.
Kidney Int, 2017 Apr 01; 92(2). PMID: 28359537
CD16+ Monocytes and Skewed Macrophage Polarization toward M2 Type Hallmark Heart Transplant Acute Cellular Rejection.
Thierry P P van den Bosch, Kadir Caliskan, +7 authors, Ajda T Rowshani.
Front Immunol, 2017 Apr 11; 8. PMID: 28392789    Free PMC article.
A Systematic Analysis of Discordant Diagnoses in Digital Pathology Compared With Light Microscopy.
Bethany J Williams, Philip DaCosta, Edward Goacher, Darren Treanor.
Arch Pathol Lab Med, 2017 May 04; 141(12). PMID: 28467215
Systematic Review.
Validation of whole-slide imaging in the primary diagnosis of liver biopsies in a University Hospital.
Adela Saco, Alba Diaz, +7 authors, Jaume Ordi.
Dig Liver Dis, 2017 Aug 07; 49(11). PMID: 28780052
Late graft dysfunction after pediatric heart transplantation is associated with fibrosis and microvasculopathy by automated, digital whole-slide analysis.
Brian Feingold, Jennifer Picarsic, +3 authors, Anthony J Demetris.
J Heart Lung Transplant, 2017 Oct 23; 36(12). PMID: 29055602
Development of CD3 cell quantitation algorithms for renal allograft biopsy rejection assessment utilizing open source image analysis software.
Andres Moon, Geoffrey H Smith, +3 authors, Alton B Brad Farris.
Virchows Arch, 2017 Nov 09; 472(2). PMID: 29116389
Global quality assessment of liver allograft C4d staining during acute antibody-mediated rejection in formalin-fixed, paraffin-embedded tissue.
Desley A H Neil, Christopher O Bellamy, +7 authors, Anthony J Demetris.
Hum Pathol, 2017 Dec 31; 73. PMID: 29288041
Digital cytology: A short review of technical and methodological approaches and applications.
A Capitanio, R E Dina, D Treanor.
Cytopathology, 2018 Apr 18; 29(4). PMID: 29665178
Review.
Deep Learning Global Glomerulosclerosis in Transplant Kidney Frozen Sections.
Jon N Marsh, Matthew K Matlock, +4 authors, S Joshua Swamidass.
IEEE Trans Med Imaging, 2018 Jul 12; 37(12). PMID: 29994669    Free PMC article.
Validation of Remote Digital Frozen Sections for Cancer and Transplant Intraoperative Services.
Luca Cima, Matteo Brunelli, +14 authors, Albino Eccher.
J Pathol Inform, 2018 Nov 20; 9. PMID: 30450263    Free PMC article.
Enhancing the Value of Histopathological Assessment of Allograft Biopsy Monitoring.
Michelle A Wood-Trageser, Andrew J Lesniak, Anthony J Demetris.
Transplantation, 2019 Feb 16; 103(7). PMID: 30768568    Free PMC article.
Review.
Physician perspectives on integration of artificial intelligence into diagnostic pathology.
Shihab Sarwar, Anglin Dent, +4 authors, Phedias Diamandis.
NPJ Digit Med, 2019 Jul 16; 2. PMID: 31304375    Free PMC article.
Morphometric image analysis and eosinophil counts in human liver allografts.
Z Ben-Ari, J D Booth, +3 authors, Burroughs.
Transpl Int, 1995 Jan 01; 8(5). PMID: 7576015
Telepathology is available for transplantation-pathology: experience in Japan using an integrated, low-cost, and high-quality system.
H Ito, H Adachi, +2 authors, K Dohi.
Mod Pathol, 1994 Sep 01; 7(7). PMID: 7824517
Monocyte chemotactic peptide-1 expression and monocyte infiltration in acute renal transplant rejection.
G Grandaliano, L Gesualdo, +3 authors, F P Schena.
Transplantation, 1997 Feb 15; 63(3). PMID: 9039933
Quantitative investigation of cardiomyocyte hypertrophy and myocardial fibrosis over 6 years after cardiac transplantation.
A T Armstrong, P F Binkley, +2 authors, C V Leier.
J Am Coll Cardiol, 1998 Sep 19; 32(3). PMID: 9741515
Whole Slide Imaging and Its Applications to Histopathological Studies of Liver Disorders.
Rossana C N Melo, Maximilian W D Raas, +3 authors, Thiago P Silva.
Front Med (Lausanne), 2020 Jan 24; 6. PMID: 31970160    Free PMC article.
Review.
Precision transplant pathology.
Michelle A Wood-Trageser, Qinyong Xu, +3 authors, Anthony J Demetris.
Curr Opin Organ Transplant, 2020 Jun 11; 25(4). PMID: 32520786    Free PMC article.
Review.
Commentary: The Digital Fate of Glomeruli in Renal Biopsy.
Ilaria Girolami, Stefano Marletta, Albino Eccher.
J Pathol Inform, 2021 May 21; 12. PMID: 34012718    Free PMC article.
Resolution-based distillation for efficient histology image classification.
Joseph DiPalma, Arief A Suriawinata, +2 authors, Saeed Hassanpour.
Artif Intell Med, 2021 Sep 18; 119. PMID: 34531005    Free PMC article.