Review
. 2020 Jul; 160:37-42.
doi: 10.1016/j.pbiomolbio.2020.05.006.

High resolution single particle Cryo-EM refinement using JSPR

Chen Sun 1 Brenda Gonzalez 1 Frank S Vago 1 Wen Jiang 2 
Affiliations
  • PMID: 32622834
  •     30 References
  •     1 citations

Abstract

JSPR is a single particle cryo-EM image processing and 3D reconstruction software developed in the Jiang laboratory at Purdue University. It began as a few refinement scripts for symmetric and asymmetric reconstructions of icosahedral viruses, but has grown into a comprehensive suite of tools for building ab initio reconstructions, high resolution refinements of viruses, protein complexes of arbitrary symmetries including helical tubes/filaments, and image file handling utilities. In this review, we will present examples achieved using JSPR and demonstrate recently implemented features of JSPR such as multi-aberration "alignments" and automatic optimization of masking for the assessment of map resolution using "true" FSC.

Keywords: Aberration correction; Beam tilt; Defocus refinement; Elliptic distortion; Generalized 2D alignments; Phase shift refinement.

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