Journal Article
. 2022 Jan; 23(1):1.
doi: 10.1186/s12865-021-00475-z.

Membrane tension sensing molecule-FNBP1 is a prognostic biomarker related to immune infiltration in BRCA, LUAD and STAD

Zixuan Wang 1 Zixin Tian 1 Xi Song 1 Jun Zhang 2 
  • PMID: 34998385
  •     48 References


Background: Formin-binding protein 1/17 (FNBP1/FBP17), as a membrane-bound protein, is wildly expressed in eukaryotic cells and performs a critical role in tumor tumorigenesis and progression. However, the relationship between FNBP1 and immune infiltrating cells, prognostic value in patients still require comprehensive understanding. We purposed to explore the correlations of FNBP1 expression, prognosis and immune infiltration levels in various cancers.

Method: The expression and survival data of FNBP1 were collected from Oncomine, TIMER, GEPIA, Kaplan-Meier Plotter and PrognoScan databases. Correlations between FNBP1 and immune infiltrates were analyzed in TIMER and GEPIA databases.

Results: Compared with normal tissues, FNBP1 is significantly differentially expressed in a variety of tumor tissues. FNBP1 has significant and complex effects on the prognosis of kinds of cancers. High-expression was obviously correlated with better prognosis in breast carcinoma and lung adenocarcinoma, while worse prognosis in stomach adenocarcinoma. Besides, FNBP1 had a correlation with various immune infiltrating cells and diverse immune gene markers in breast invasive carcinoma (BRCA), lung adenocarcinoma (LUAD), and stomach adenocarcinoma (STAD). FNBP1 was also positively correlated with the adjustment of CD8+ cells, T cells, M2 macrophage, neutrophils, monocyte, Th1 cells, T regulatory cells (Treg) and Tumor-associated macrophages (TAMs). The expression level of FNBP1 is closely positively correlated with the expression level of multiple immune checkpoints in the three cancers. In addition, FNBP1 is significantly positively correlated with the expression levels of a variety of immunosuppressive molecules.

Conclusion: Our findings reveal FNBP1 can serve as a significant biomarker to influence the prognosis and the immune infiltrating levels in different cancers. The differential expression of FNBP1 might not only contribute to the judgment of metastatic and non-metastatic tumors but also in the immune escape by upregulating the expression of immune checkpoints.

TIMER: A Web Server for Comprehensive Analysis of Tumor-Infiltrating Immune Cells.
Taiwen Li, Jingyu Fan, +5 authors, X Shirley Liu.
Cancer Res, 2017 Nov 03; 77(21). PMID: 29092952    Free PMC article.
Highly Cited.
Lymphocyte-activation gene 3 (LAG3): The next immune checkpoint receptor.
Elisa Ruffo, Richard C Wu, +2 authors, Dario A A Vignali.
Semin Immunol, 2019 Oct 13; 42. PMID: 31604537    Free PMC article.
The human formin-binding protein 17 (FBP17) interacts with sorting nexin, SNX2, and is an MLL-fusion partner in acute myelogeneous leukemia.
U Fuchs, G Rehkamp, +8 authors, A Borkhardt.
Proc Natl Acad Sci U S A, 2001 Jul 05; 98(15). PMID: 11438682    Free PMC article.
An online survival analysis tool to rapidly assess the effect of 22,277 genes on breast cancer prognosis using microarray data of 1,809 patients.
Balazs Györffy, Andras Lanczky, +4 authors, Zoltan Szallasi.
Breast Cancer Res Treat, 2009 Dec 19; 123(3). PMID: 20020197
Highly Cited.
CellMarker: a manually curated resource of cell markers in human and mouse.
Xinxin Zhang, Yujia Lan, +14 authors, Yun Xiao.
Nucleic Acids Res, 2018 Oct 06; 47(D1). PMID: 30289549    Free PMC article.
Highly Cited.
KEGG: integrating viruses and cellular organisms.
Minoru Kanehisa, Miho Furumichi, +2 authors, Mao Tanabe.
Nucleic Acids Res, 2020 Oct 31; 49(D1). PMID: 33125081    Free PMC article.
High expression of FBP17 in invasive breast cancer cells promotes invadopodia formation.
Prabhat Suman, Sarthak Mishra, Harish Chander.
Med Oncol, 2018 Apr 14; 35(5). PMID: 29651632
MSF (MLL septin-like fusion), a fusion partner gene of MLL, in a therapy-related acute myeloid leukemia with a t(11;17)(q23;q25).
M Osaka, J D Rowley, N J Zeleznik-Le.
Proc Natl Acad Sci U S A, 1999 May 26; 96(11). PMID: 10339604    Free PMC article.
Use the force: membrane tension as an organizer of cell shape and motility.
Alba Diz-Muñoz, Daniel A Fletcher, Orion D Weiner.
Trends Cell Biol, 2012 Nov 06; 23(2). PMID: 23122885    Free PMC article.
Highly Cited. Review.
NK Cell Metabolism and Tumor Microenvironment.
Iñigo Terrén, Ane Orrantia, +2 authors, Francisco Borrego.
Front Immunol, 2019 Oct 17; 10. PMID: 31616440    Free PMC article.
Prognostic value of prostaglandin I2 synthase and its correlation with tumor-infiltrating immune cells in lung cancer, ovarian cancer, and gastric cancer.
Danian Dai, Bo Chen, +4 authors, Jihong Liu.
Aging (Albany NY), 2020 May 29; 12(10). PMID: 32463792    Free PMC article.
GEPIA: a web server for cancer and normal gene expression profiling and interactive analyses.
Zefang Tang, Chenwei Li, +3 authors, Zemin Zhang.
Nucleic Acids Res, 2017 Apr 14; 45(W1). PMID: 28407145    Free PMC article.
Highly Cited.
The Role of Surgical Pathology in Guiding Cancer Immunotherapy.
Scott B Lovitch, Scott J Rodig.
Annu Rev Pathol, 2016 May 20; 11. PMID: 27193453
Requirement for FBP17 in invadopodia formation by invasive bladder tumor cells.
Hayato Yamamoto, Mihoko Sutoh, +9 authors, Shigeru Tsuboi.
J Urol, 2011 Mar 23; 185(5). PMID: 21421245
Sp1-Induced FNBP1 Drives Rigorous 3D Cell Motility in EMT-Type Gastric Cancer Cells.
Bo Kyung Yoon, Nahee Hwang, +7 authors, Jae-Woo Kim.
Int J Mol Sci, 2021 Jul 03; 22(13). PMID: 34202606    Free PMC article.
Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources.
Da Wei Huang, Brad T Sherman, Richard A Lempicki.
Nat Protoc, 2009 Jan 10; 4(1). PMID: 19131956
Highly Cited.
FBP17 Mediates a Common Molecular Step in the Formation of Podosomes and Phagocytic Cups in Macrophages.
Shigeru Tsuboi, Hidetoshi Takada, +8 authors, Hans D Ochs.
J Biol Chem, 2009 Jan 22; 284(13). PMID: 19155218    Free PMC article.
PD-1 and its ligands in tolerance and immunity.
Mary E Keir, Manish J Butte, Gordon J Freeman, Arlene H Sharpe.
Annu Rev Immunol, 2008 Jan 05; 26. PMID: 18173375
Highly Cited. Review.
The extracellular matrix in tumor progression and metastasis.
Johannes A Eble, Stephan Niland.
Clin Exp Metastasis, 2019 Apr 12; 36(3). PMID: 30972526
Highly Cited. Review.
Translating Membrane Tension into Cytoskeletal Action by FBP17.
Mirjam M Zegers, Peter Friedl.
Dev Cell, 2015 Jun 24; 33(6). PMID: 26102599
Microenvironmental regulation of tumor progression and metastasis.
Daniela F Quail, Johanna A Joyce.
Nat Med, 2013 Nov 10; 19(11). PMID: 24202395    Free PMC article.
Highly Cited. Review.
Metastatic colonization by circulating tumour cells.
Joan Massagué, Anna C Obenauf.
Nature, 2016 Jan 23; 529(7586). PMID: 26791720    Free PMC article.
Highly Cited. Review.
Coordination between the actin cytoskeleton and membrane deformation by a novel membrane tubulation domain of PCH proteins is involved in endocytosis.
Kazuya Tsujita, Shiro Suetsugu, +3 authors, Tadaomi Takenawa.
J Cell Biol, 2006 Jan 19; 172(2). PMID: 16418535    Free PMC article.
Highly Cited.
Systematic pan-cancer analysis of tumour purity.
Dvir Aran, Marina Sirota, Atul J Butte.
Nat Commun, 2015 Dec 05; 6. PMID: 26634437    Free PMC article.
Highly Cited.
CFP is a prognostic biomarker and correlated with immune infiltrates in Gastric Cancer and Lung Cancer.
Guoliang Cui, Le Geng, +6 authors, Fei Wei.
J Cancer, 2021 May 13; 12(11). PMID: 33976747    Free PMC article.
Feedback regulation between plasma membrane tension and membrane-bending proteins organizes cell polarity during leading edge formation.
Kazuya Tsujita, Tadaomi Takenawa, Toshiki Itoh.
Nat Cell Biol, 2015 May 06; 17(6). PMID: 25938814
Oncomine 3.0: genes, pathways, and networks in a collection of 18,000 cancer gene expression profiles.
Daniel R Rhodes, Shanker Kalyana-Sundaram, +10 authors, Arul M Chinnaiyan.
Neoplasia, 2007 Mar 16; 9(2). PMID: 17356713    Free PMC article.
Highly Cited.
The WASP-WAVE protein network: connecting the membrane to the cytoskeleton.
Tadaomi Takenawa, Shiro Suetsugu.
Nat Rev Mol Cell Biol, 2006 Dec 22; 8(1). PMID: 17183359
Highly Cited. Review.
Ultrastructure of the actin cytoskeleton.
Tatyana M Svitkina.
Curr Opin Cell Biol, 2018 Feb 25; 54. PMID: 29477121    Free PMC article.
Chimeric MLL products with a Ras binding cytoplasmic protein AF6 involved in t(6;11) (q27;q23) leukemia localize in the nucleus.
T Joh, K Yamamoto, +7 authors, M Seto.
Oncogene, 1998 Feb 12; 15(14). PMID: 9349501
Covalent dimerization of CD28/CTLA-4 and oligomerization of CD80/CD86 regulate T cell costimulatory interactions.
J L Greene, G M Leytze, +4 authors, P S Linsley.
J Biol Chem, 1996 Oct 25; 271(43). PMID: 8900156
t(11;22)(q23;q11.2) In acute myeloid leukemia of infant twins fuses MLL with hCDCrel, a cell division cycle gene in the genomic region of deletion in DiGeorge and velocardiofacial syndromes.
M D Megonigal, E F Rappaport, +7 authors, C A Felix.
Proc Natl Acad Sci U S A, 1998 May 30; 95(11). PMID: 9600980    Free PMC article.
The Actin Cytoskeleton and Actin-Based Motility.
Tatyana Svitkina.
Cold Spring Harb Perspect Biol, 2018 Jan 04; 10(1). PMID: 29295889    Free PMC article.
Highly Cited. Review.
Dynamic organization of the actin system in the motile cells of Dictyostelium.
Till Bretschneider, James Jonkman, +6 authors, Günther Gerisch.
J Muscle Res Cell Motil, 2003 Sep 04; 23(7-8). PMID: 12952063
Bioinformatics enrichment tools: paths toward the comprehensive functional analysis of large gene lists.
Da Wei Huang, Brad T Sherman, Richard A Lempicki.
Nucleic Acids Res, 2008 Nov 27; 37(1). PMID: 19033363    Free PMC article.
Highly Cited.
Toward understanding the origin and evolution of cellular organisms.
Minoru Kanehisa.
Protein Sci, 2019 Aug 24; 28(11). PMID: 31441146    Free PMC article.
Highly Cited. Review.
Immune inhibitory molecules LAG-3 and PD-1 synergistically regulate T-cell function to promote tumoral immune escape.
Seng-Ryong Woo, Meghan E Turnis, +18 authors, Dario A A Vignali.
Cancer Res, 2011 Dec 22; 72(4). PMID: 22186141    Free PMC article.
Highly Cited.
TIM3 comes of age as an inhibitory receptor.
Yochai Wolf, Ana C Anderson, Vijay K Kuchroo.
Nat Rev Immunol, 2019 Nov 05; 20(3). PMID: 31676858    Free PMC article.
Fc-dependent depletion of tumor-infiltrating regulatory T cells co-defines the efficacy of anti-CTLA-4 therapy against melanoma.
Tyler R Simpson, Fubin Li, +11 authors, Sergio A Quezada.
J Exp Med, 2013 Jul 31; 210(9). PMID: 23897981    Free PMC article.
Highly Cited.
Basic Overview of Current Immunotherapy Approaches in Cancer.
Vamsidhar Velcheti, Kurt Schalper.
Am Soc Clin Oncol Educ Book, 2016 Jun 02; 35. PMID: 27249709
Comprehensive analyses of tumor immunity: implications for cancer immunotherapy.
Bo Li, Eric Severson, +10 authors, X Shirley Liu.
Genome Biol, 2016 Aug 24; 17(1). PMID: 27549193    Free PMC article.
Highly Cited.
Xanthine dehydrogenase as a prognostic biomarker related to tumor immunology in hepatocellular carcinoma.
Zhen Lin, Yi-Zhao Xie, +3 authors, Guang-Liang Chen.
Cancer Cell Int, 2021 Sep 10; 21(1). PMID: 34496841    Free PMC article.
KEGG: kyoto encyclopedia of genes and genomes.
M Kanehisa, S Goto.
Nucleic Acids Res, 1999 Dec 11; 28(1). PMID: 10592173    Free PMC article.
Highly Cited.
Fates of CD8+ T cells in Tumor Microenvironment.
Nomathamsanqa Resegofetse Maimela, Shasha Liu, Yi Zhang.
Comput Struct Biotechnol J, 2018 Dec 26; 17. PMID: 30581539    Free PMC article.
Highly Cited. Review.
CD155/TIGIT, a novel immune checkpoint in human cancers (Review).
Lu Liu, Xuewu You, +3 authors, Youzhong Zhang.
Oncol Rep, 2021 Jan 21; 45(3). PMID: 33469677
PD-1 regulates germinal center B cell survival and the formation and affinity of long-lived plasma cells.
Kim L Good-Jacobson, Courtney G Szumilas, +3 authors, Mark J Shlomchik.
Nat Immunol, 2010 May 11; 11(6). PMID: 20453843    Free PMC article.
Highly Cited.
Tumor-infiltrating NY-ESO-1-specific CD8+ T cells are negatively regulated by LAG-3 and PD-1 in human ovarian cancer.
Junko Matsuzaki, Sacha Gnjatic, +9 authors, Kunle Odunsi.
Proc Natl Acad Sci U S A, 2010 Apr 14; 107(17). PMID: 20385810    Free PMC article.
Highly Cited.
ONCOMINE: a cancer microarray database and integrated data-mining platform.
Daniel R Rhodes, Jianjun Yu, +6 authors, Arul M Chinnaiyan.
Neoplasia, 2004 Apr 08; 6(1). PMID: 15068665    Free PMC article.
Highly Cited.