Segmentation of large areas of light microscopic slides into N by N fields, and each of these fields into M digital image tiles, allows the scanning, storage and digital processing of large images. Any of the original N2 fields or composites of M adjacent tiles can be recalled to the video display for analysis. Developed procedures for use on a microscope equipped with a precision scanning stage allow registration of the image coordinates (X-Y) for any original or composite field and the alignment of one of these fields along the depth (Z) axis by means of external, machined fiducial marks in serial sections. To facilitate work whenever unavoidable, we have incorporated methods for digital image panning and zooming (changes of magnification) and discuss their use and implications.
Comparison between digital and optical microscopy: Analysis in a mouse gut inflammation model.
Biomed Rep, 2017 Sep 13; 7(3). PMID: 28894572 Free PMC article.
From Scope to Screen: The Evolution of Histology Education.
Adv Exp Med Biol, 2020 Nov 20; 1260. PMID: 33211308