In the following article a method is described for the distortion-free reassemblage of adjacent digitised microscopic images of histological sections. The correct positioning of the microscopic specimen is achieved via a computer-controlled scanning stage. During an internal calibration step the correction function for the image distortions and the parameters of the transformation scanning-stage-to image-coordinate system are determined. In the subsequent application phase the new positions the scanning stage has to move to can now be computed in such a way that the distortion-corrected digitised images of the section parts under consideration neatly fit together to one composite image.
Comparison between digital and optical microscopy: Analysis in a mouse gut inflammation model.
Biomed Rep, 2017 Sep 13; 7(3). PMID: 28894572 Free PMC article.